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Cat. No.: HY-171995

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Rf470 TFA

(Synonyms: Rotor NO.4 TFA)

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Rf470 (Rotor NO.4) TFA is a FMR-probe-D-lysine conjugate (Max Ex: 470 nM; Max Em: 640 nM) that can be covalently incorporated into peptidoglycan by bacteria. Rf470 TFA has extremely weak fluorescence in the free state, but its fluorescence is significantly enhanced when it is catalytically incorporated into peptidoglycan by transpeptidases. Through fluorescence changes, Rf470 TFA can be used for real-time monitoring of peptidoglycan biosynthesis, detection of transpeptidase activity, and screening of antibiotics, etc.

For research use only. We do not sell to patients.

CAS No.: 2369899-34-1
Formula: C₂₈H₃₁F₃N₄O₇S
Molecular Weight:624.63

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Data Sheet (266 KB)

Rf470 TFA Chemical Structure

CAS No.: 2369899-34-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Chemical Information

CAS No. 2369899-34-1
Appearance Solid
Molecular Weight 624.63
Formula C₂₈H₃₁F₃N₄O₇S
SMILES

OC(C(F)(F)F)=O.OC([C@H](N)CCCCNC(CCN1C2=CC=C(C3=CC=C(/C=C(C#N)/C(O)=O)S3)C=C2CCC1)=O)=O
Synonyms

Rotor NO.4 TFA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Michael VanNIEUWENHZE, et al. Molecular rotor-based d-amino acids as tools for imaging peptidoglycan biosynthesis. WO2019157233A1. 2019-08-15.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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