NSC 689534 

NSC 689534 can form copper chelate with Cu²⁺. NSC 689534/Cu²⁺ complex is a potent oxidative stress inducer, and has antitumor activity^[1].

NSC 689534/Cu²⁺ complex (48 h) inhibits cell viability of HL60 and PC3 cell with IC₅₀s of 0.2 and 0.4 μM respectively, which is about 4 times more potent than NSC 689534 alone^[1].
NSC 689534/Cu²⁺ complex (2.5 μM, 24 h) induces oxidative stress and depletes GSH in PC3 cells, whereas with no effect by NSC 689534 alone^[1].
NSC 689534/Cu²⁺ complex (2.5 μM, 24 h) induces macroautophagy (indicated by LC3 accumulation into large autophagosomes) and an ER-stress response (upregulation of GRP78 and CHOP) in PC3 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NSC 689534/Cu²⁺ complex (3 mg/kg, i.p., once or twice a day for 5 days) inhibits tumor growth in HL60 xenograft model, whereas with no statistical significance by NSC 689534 alone^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

362.45

C₁₉H₁₈N₆S

907958-80-9

Solid

Light yellow to yellow

S=C(N(CC1=NC=CC=C1)CC2=NC=CC=C2)N/N=C/C3=NC=CC=C3

Room temperature in continental US; may vary elsewhere.

• [1]. Chad N Hancock, et al. A copper chelate of thiosemicarbazone NSC 689534 induces oxidative/ER stress and inhibits tumor growth in vitro and in vivo. Free Radic Biol Med. 2011 Jan 1;50(1):110-21.  [Content Brief]

  [2]. Hancock CN, et al. A copper chelate of thiosemicarbazone NSC 689534 induces oxidative/ER stress and inhibits tumor growth in vitro and in vivo. Free Radic Biol Med. 2011 Jan 1;50(1):110-21.  [Content Brief]