Peimisine hydrochloride
Based on 3 publication(s) in Google Scholar
Peimisine (Ebeiensine) hydrochloride is a muscarinic M receptor antagonist and angiotensin converting enzyme (ACE) inhibitor. Peimisine hydrochloride shows anti-tumor, anti-inflammatory, antihypertensive activities. Peimisine can induce apoptosis and be used in cough and asthma research.
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- Pureté : 99.34%
- CAS No.: 900498-44-4
- Formule: C27H42ClNO3
- Masse moléculaire:464.08
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Peimisine hydrochloride
More
Customer Validation & Images
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In Vivo Efficacy Study
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Histological Imaging/Staining
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WB
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IF
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ELISA
Activité biologique
Description
In Vitro
Peimisine (17.43-92.07 μg/mL; 72 h) shows significant cytotoxic effects[3].
Peimisine (15 μg/mL; 24, 48 and 72 h) induces G0/G1 phase arrest and rising apoptosis rate[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A2780 cells
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Concentration:15 μg/mL
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Incubation Time:24, 48 and 72 hours
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Result:Induced G0/G1 phase arrest of A2780 cells in a time-dependent manner.
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Cell Line:LLC, A2780, HepG2 and A549 cells
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Concentration:17.43-92.07 μg/mL
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Incubation Time:72 hours
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Result:Inhibited LLC, A2780, HepG2 and A549 cells with the IC50 values of 20.75 μg/mL, 17.43 μg/mL, 92.07 μg/mL, 36.11 μg/mL, respectively.
Chemical Information
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CAS No. 900498-44-4
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Appearance Solid
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Masse moléculaire 464.08
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Formule C27H42ClNO3
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Color White to off-white
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SMILES
CC1=C(C[C@@]2([H])[C@@]3([H])CC([C@]4([H])[C@@]2(CC[C@@H](C4)O)C)=O)[C@@]3([H])CC[C@]15O[C@@]6([H])[C@](NC[C@H](C6)C)([H])[C@H]5C.[H]Cl
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Synonyms
Ebeiensine hydrochloride
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Total alkaloids of Fritillaria unibracteata var. wabuensis bulbus ameliorate chronic asthma via the TRPV1/Ca2+/NFAT pathway. [Abstract]2023 Sep:118:154946. PMID: 37421766 -
Molecules
Metabolite Profiling and Anti-Inflammatory Activities of Fritillaria cirrhosa D. Don Bulbs Derived from Tissue Culture. [Abstract]2025 Jan 31;30(3):623. PMID: 39942727 -
J Pharm Pharmacol
Peimisine ameliorates DSS-induced colitis by suppressing Jak-Stat activation and alleviating gut microbiota dysbiosis in mice. [Abstract]2024 May 3;76(5):545-558. PMID: 38007392
Peimisine hydrochloride purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Colon length treated with Peimisine (5, 15 mg/kg).
Peimisine hydrochloride purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Representative images of HE staining of colon tissues and histological scores based on HE-stained sections treated with Peimisine (5, 15 mg/kg).
Peimisine hydrochloride purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Western-blot analysis of IL-17A, FoxP3, and IL-17A/FoxP3 expression in mice colon treated with Peimisine (5, 15 mg/kg).
Peimisine hydrochloride purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Immunofluorescence assessment of intestinal IL-17 (red) and FoxP3 (green) treated with Peimisine (5, 15 mg/kg).
Peimisine hydrochloride purchased from MedChemExpress. Usage Cited in: J Pharm Pharmacol. 2024 May 3;76(5):545-558. [Abstract]
Expression levels of inflammatory cytokines MCP-1, TNF-α, IL-6, IL-1β, and IFN-γ in serum treated with Peimisine (5, 15 mg/kg).
Solvant et solubilité
In Vitro:
DMSO : 7.14 mg/mL (15.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.71 mg/mL (1.53 mM); Clear solution
This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.71 mg/mL (1.53 mM); Clear solution
This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Pan F, et al. Peimisine and peiminine production by endophytic fungus Fusarium sp. isolated from Fritillaria unibracteata var. wabensis. Phytomedicine. 2014 Jul-Aug;21(8-9):1104-9. [Content Brief]
[2]. Armando Alberola-Die, et al. Peimine, an Anti-Inflammatory Compound from Chinese Herbal Extracts, Modulates Muscle-Type Nicotinic Receptors. Int J Mol Sci. 2021 Oct 19;22(20):11287. [Content Brief]
[3]. Dongdong Wang, et al. Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action. BMC Complement Altern Med. 2015 Feb 21;15:29. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1548 mL | 10.7740 mL | 21.5480 mL | 53.8700 mL |
| 5 mM | 0.4310 mL | 2.1548 mL | 4.3096 mL | 10.7740 mL | |
| 10 mM | 0.2155 mL | 1.0774 mL | 2.1548 mL | 5.3870 mL | |
| 15 mM | 0.1437 mL | 0.7183 mL | 1.4365 mL | 3.5913 mL |
Peimisine hydrochloride Related Classifications
HY-N0214A Related Classifications
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