Pep-1-Cysteamine 

Pep-1-Cysteamine is an amphipathic chimeric cell-penetrating peptide. Pep-1-Cysteamine can penetrate biological membranes in an energy-independent manner without forming transmembrane pores, and efficiently deliver active proteins into cells, with its translocation dominated by electrostatic interactions and membrane perturbation^[1].

When incorporated into lipid multilamellar membranes, Pep-1-Cysteamine shows an increased proportion of α-helical secondary structure compared to its random coil conformation^[1].
Pep-1-Cysteamine (0-4 μM; 0-30 min) does not induce pore formation in POPC or POPC:POPG giant unilamellar vesicles (GUVs) with a molar ratio of 4:1; instead, it triggers complete membrane disruption via a detergent-like mechanism at high concentrations (≥0.9 μM for POPC:POPG, 2-4 μM for POPC), accompanied by total dye leakage^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2949.39

C₁₄₀H₂₀₂N₃₆O₃₃S

863608-35-9

Solid

White to off-white

Ac-Lys-Glu-Thr-Trp-Trp-Glu-Thr-Trp-Trp-Thr-Glu-Trp-Ser-Gln-Pro-Lys-Lys-Lys-Arg-Lys-Val-cysteamine

Ac-KETWWETWWTEWSQPKKKRKV-cysteamine

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Henriques ST, et al. Energy-independent translocation of cell-penetrating peptides occurs without formation of pores. A biophysical study with pep-1. Mol Membr Biol. 2007;24(4):282-293.  [Content Brief]