Phidianidine B
Phidianidine B is a highly cytotoxic 1,2,4-oxadiazole marine metabolite. Phidianidine B is a natural product that can be isolated from a marine mollusk.
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- CAS. Nr.: 1301638-42-5
- Formel: C17H23N7O
- Molecular Weight:341.41
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 1301638-42-5
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Molecular Weight 341.41
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Formel C17H23N7O
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SMILES
NC(NCCCCCNC1=NOC(CC2=CNC3=CC=CC=C23)=N1)=N
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Structure Classification
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Initial Source
marine opisthobranch mollusk Phidiana militaris
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)