Pronase E (Activity ≥ 7000 U/g)
Based on 79 publication(s) in Google Scholar
Pronase E (Activity ≥ 7000 U/g) is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus and could digest protein into individual amino acids.
For research use only. We do not sell to patients.
- CAS No.: 9036-06-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Pronase E (Activity ≥ 7000 U/g)
More- Nat Metab. 2022 Oct;4(10):1306-1321. [Abstract]
- Autophagy. 2026 May 25:1-21. [Abstract]
- Autophagy. 2026 Mar;22(3):526-544. [Abstract]
- Autophagy. 2025 Jun;21(6):1316-1334. [Abstract]
- Autophagy. 2023 Aug;19(8):2240-2256. [Abstract]
- Nat Commun. 2026 Jul 23.
- J Adv Res. 2025 Jul 14:S2090-1232(25)00544-2. [Abstract]
- Redox Biol. 2026 Mar:90:104055. [Abstract]
- Acta Pharm Sin B. 2026 Jul;16(7):4410-4425. [Abstract]
- Adv Sci (Weinh). 2026 Jun 19:e76165. [Abstract]
- Adv Sci (Weinh). 2026 Feb 28:e21818. [Abstract]
- Cell Rep Med. 2026 Jun 16;7(6):102826. [Abstract]
- Sci Adv. 2023 Jul 21;9(29):eadh4054. [Abstract]
- Pharmacol Res. 2024 Dec:210:107516. [Abstract]
- Cell Commun Signal. 2024 Oct 11;22(1):488. [Abstract]
- Phytomedicine. 2026 Sep:159:158502.
- Phytomedicine. 2026 Jul:156:158224. [Abstract]
- Phytomedicine. 2026 Jun:155:158157. [Abstract]
- Phytomedicine. 2026 Jan:150:157584. [Abstract]
- Phytomedicine. 2025 Nov 25:148:157395. [Abstract]
- Phytomedicine. 2025 Oct:146:157126. [Abstract]
- Phytomedicine. 2025 Sep:145:156994. [Abstract]
- Phytomedicine. 2024 Dec:135:156139. [Abstract]
- Phytomedicine. 2024 Oct:133:155947. [Abstract]
- J Pharm Anal. 2025 Dec;15(12):101301. [Abstract]
- Antioxidants (Basel). 2026 Apr 24;15(5):543. [Abstract]
- Antioxidants (Basel). 2026 Mar 1;15(3):313. [Abstract]
- Antioxidants (Basel). 2024 Jun 2;13(6):684. [Abstract]
- Phytother Res. 2026 May;40(5):2270-2287. [Abstract]
- Free Radic Biol Med. 2026 Jul 15:255:160-178. [Abstract]
- Am J Chin Med. 2026;54(1):329-348. [Abstract]
- Br J Pharmacol. 2025 Jun;182(12):2730-2753. [Abstract]
- Chin Med. 2026 Jul 8;21(1):185.
- J Med Chem. 2024 Nov 14;67(21):19874-19888. [Abstract]
- J Ethnopharmacol. 2025 Apr 9:345:119618. [Abstract]
- J Ethnopharmacol. 2025 Jan 10:336:118721. [Abstract]
- J Agric Food Chem. 2026 Feb 11;74(5):4420-4434. [Abstract]
- J Agric Food Chem. 2025 Dec 25. [Abstract]
- J Agric Food Chem. 2025 Sep 24;73(38):24055-24075. [Abstract]
- CNS Neurosci Ther. 2025 Sep;31(9):e70609. [Abstract]
- Biochem Pharmacol. 2026 Sep;251(Pt 1):118103. [Abstract]
- Biochem Pharmacol. 2026 Jun 20;251(Pt 2):118181. [Abstract]
- Biochem Pharmacol. 2026 May:247:117771. [Abstract]
- Biochem Pharmacol. 2025 Apr:234:116801. [Abstract]
- Life Sci. 2025 Jun 1:370:123568. [Abstract]
- Life Sci. 2024 Sep 15:353:122918. [Abstract]
- Int J Pharm. 2026 Mar 10:692:126628. [Abstract]
- J Gastroenterol. 2024 Nov;59(11):1037-1051. [Abstract]
- Eur J Pharmacol. 2024 Mar 15:967:176367. [Abstract]
- Eur J Pharmacol. 2023 Nov 15:959:176105. [Abstract]
- Int Immunopharmacol. 2026 Jun 26:186:117074. [Abstract]
- Int J Mol Sci. 2026 Mar 12;27(6):2615. [Abstract]
- Int Immunopharmacol. 2026 Feb 15:171:116095. [Abstract]
- Int J Mol Sci. 2024 Sep 10;25(18):9763. [Abstract]
- Int Immunopharmacol. 2024 Feb 15:128:111459. [Abstract]
- ACS Omega. 2026 Jun 17.
- Microchem J. 2026 Mar 31;224:117898.
- ACS Omega. 2026 Feb 19;11(8):13768-13788. [Abstract]
- Chem Biol Interact. 2025 Nov 1:421:111748. [Abstract]
- Chem Biol Interact. 2025 Sep 5:418:111578. [Abstract]
- Int J Antimicrob Agents. 2025 Aug 23:107601. [Abstract]
- Sci Rep. 2025 Sep 1;15(1):32112. [Abstract]
- Virol Sin. 2026 Jul 9:S1995-820X(26)00105-7.
- Aquaculture. 2025 May 1.
- FASEB J. 2026 Mar 31;40(6):e71698. [Abstract]
- Food Biophys. 2025 Aug 6.
- ACS Chem Biol. 2023 May 19;18(5):1168-1179. [Abstract]
- Mol Biol Rep. 2026 Jul 9;53(1):1126.
- Dig Dis Sci. 2025 Nov 8. [Abstract]
- Toxicon. 2025 Jul:261:108371. [Abstract]
- Traditional Medicine Research. 2026;11(11):72.
- SSRN. 2025 Oct 9.
- University of Alabama. 2025.
- University of California, Riverside. 2024.
- bioRxiv. 2024 September 08.
- University of East Anglia. 2024 Apr.
- SSRN. 2023 Jul 31.
- SSRN. 2023 Mar 13.
- Research Square Print. 2022 Aug.
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Biological Activity
Description
In Vitro
Pronase E significantly increases the amount of most of the amino acids analysed (PE vs C) , especially Ile, His and Thr[1]. Pronase E Usage Steps:. 1. Preparation of Stock Solution: (1) Dissolve Pronase E in deionized water to prepare a stock solution at a concentration of 5-20 mg/ml. Note: If used for DNA or RNA isolation steps, it is recommended to first heat the solution at 56°C for 15 minutes. (2) Incubate at 37°C for 1 hour. Aliquot the prepared stock solution into single-use samples and store at -20°C; typically stable for one year. 2. DNA Isolation: Directly add Pronase E to the DNA preparation system. The system should contain 0.5-1% SDS to disrupt DNA-protein interactions. Note: Use at a concentration of 250-500 μg protein/ml. Incubation conditions: Incubate at 37°C for 1-4 hours. 3. Protein Digestion: (1) Dissolve approximately 0.2 μM protein in 0.2 ml of 50 mM ammonium bicarbonate buffer (pH 8.0) or phosphate buffer (pH 7.0). (2) Add 1% (w/w) Pronase E. Incubate at 37°C for 24 hours to perform protein digestion.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 9036-06-0
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Pronase E (Activity?7000 U/g)]
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Synonyms
Pronase (Activity ≥ 7000 U/g)
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Structure Classification
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Initial Source
Streptomyces griseus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (79)
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Journal Impact Factor
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Most Recent
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Nat Metab
HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma. [Abstract]2022 Oct;4(10):1306-1321. PMID: 36192599 -
Autophagy
Byakangelicin alleviates metabolic dysfunction-associated steatohepatitis by selective inhibition of a non-canonical MTORC1 signaling pathway. [Abstract]2026 May 25:1-21. PMID: 42152469 -
Autophagy
Lactucopicrin promotes the autophagic degradation of MAP2K4/MKK4 by mediating CCDC50 palmitoylation to alleviate osteoarthritis progression. [Abstract]2026 Mar;22(3):526-544. PMID: 41566717 -
Autophagy
Buddleoside alleviates nonalcoholic steatohepatitis by targeting the AMPK-TFEB signaling pathway. [Abstract]2025 Jun;21(6):1316-1334. PMID: 39936600 -
Autophagy
Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin. [Abstract]2023 Aug;19(8):2240-2256. PMID: 36779633 -
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J Adv Res
Tussilagone attenuated cigarette smoke-induced chronic obstructive pulmonary disease through regulating Nrf2 and NF-κB/NLRP3 inflammasome via directly targeting cysteine 434 of KEAP1. [Abstract]2025 Jul 14:S2090-1232(25)00544-2. PMID: 40669599 -
Redox Biol
DMNQ induces ferroptosis and augments the efficacy of anti-PD-L1 immunotherapy in gastric cancer via the STAT3/SLC1A4 axis to mediate cysteine metabolism reprogramming. [Abstract]2026 Mar:90:104055. PMID: 41604939 -
Acta Pharm Sin B
Insights into SARS-CoV-2 replication control via targeting the host hijacking function of NSP12. [Abstract]2026 Jul;16(7):4410-4425. PMID: 42453420 -
Adv Sci (Weinh)
Targeting the HSPA8-CMA-ATP6V1A Axis Triggers Lysosomal Hyperacidification and Catastrophic Vacuolation in Prostate Cancer. [Abstract]2026 Jun 19:e76165. PMID: 42318659 -
Adv Sci (Weinh)
Artesunate Ameliorates APAP-induced Liver Injury by Promoting NEDD4L-Mediated Ubiquitination and Degradation of TXNIP. [Abstract]2026 Feb 28:e21818. PMID: 41762705 -
Cell Rep Med
Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models. [Abstract]2026 Jun 16;7(6):102826. PMID: 42167246 -
Sci Adv
2023 Jul 21;9(29):eadh4054. PMID: 37478191 -
Pharmacol Res
Gentisic acid prevents the development of atherosclerotic lesions by inhibiting SNX10-mediated stabilization of LRP6. [Abstract]2024 Dec:210:107516. PMID: 39603572
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Dec:210:107516. [Abstract]
Cell lysis was incubated with GA (0, 12.5, 25, and 50 μM) and then subjected to Pronase E (20 μg/mL; 30 min) digestion. The protein level of SNX10 was measured by Western Blot and quantified by Image J software.
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Cell Commun Signal
Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling. [Abstract]2024 Oct 11;22(1):488. PMID: 39394127 -
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Phytomedicine
Buyang Huanwu Decoction suppresses atherosclerosis by targeting IFITM1‑mediated lipid metabolism in vascular smooth muscle cells. [Abstract]2026 Jul:156:158224. PMID: 42030801 -
Phytomedicine
Schisantherin A alleviates DSS-induced colitis by upregulating ferredoxin 1 to inhibit inflammation and restore intestinal barrier function. [Abstract]2026 Jun:155:158157. PMID: 41966028 -
Phytomedicine
Licochalcone B targets neural cell adhesion molecule 1 to inhibit osteosarcoma progression and ferroptosis resistance via extracellular signal-regulated kinase 5 and nuclear factor kappa B pathways. [Abstract]2026 Jan:150:157584. PMID: 41349457
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jan:150:157584. [Abstract]
Pronase E (1:2000-1000; 30 min) and Licochalcone B (20 μM; 30 min). The NCAM1 protein expression of drug affinity responsive target stability assay (upper panel) and statistical analysis (lower panel). Ns, not significant. **, P <0.01; ***, P <0.001; ****, P <0.0001.
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Phytomedicine
Guben-Qushi-Huayu formula and calycosin improve epidermal barrier function in psoriasis by inhibiting small proline-rich protein 1A. [Abstract]2025 Nov 25:148:157395. PMID: 41166908 -
Phytomedicine
Dihydrocapsaicin attenuates oxidative stress and apoptosis in acute myocardial infarction via promoting Raf-1/ASK1 complex formation. [Abstract]2025 Oct:146:157126. PMID: 40768809 -
Phytomedicine
Tanshinone I alleviates post-ischemic myocardial injury by targeting TGFBR1 and modulating the TGF-β signaling pathway. [Abstract]2025 Sep:145:156994. PMID: 40580691
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:156994. [Abstract]
Effect of Tan I on TGFBR1 in Pronase E (500:1; 1h)-treated H9c2 cells.
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Phytomedicine
Shikonin induces ferroptosis in osteosarcomas through the mitochondrial ROS-regulated HIF-1α/HO-1 axis. [Abstract]2024 Dec:135:156139. PMID: 39423479 -
Phytomedicine
Silymarin targets the FXR protein through microbial metabolite 7-keto-deoxycholic acid to treat MASLD in obese mice. [Abstract]2024 Oct:133:155947. PMID: 39178642
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Oct:133:155947. [Abstract]
Pronase E (3 ug; 25 min) degrades FXR and β-actin by 1.5%. DARTS results of 7-KDCA on FXR.
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J Pharm Anal
α-hederin decreases the glycolysis level in intestinal epithelial cells via SNX10-mediated DEPDC5 degradation. [Abstract]2025 Dec;15(12):101301. PMID: 41487148
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec;15(12):101301. [Abstract]
Pronase E (25 μg/mL; 30 min). The binding of α-hed with SNX10 and DEPDC5 was determined by drug affinity responsive target stability (DARTS) assay.
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Antioxidants (Basel)
Empagliflozin Ameliorates Diabetic Cardiomyopathy by Inhibiting Ferroptosis via SIRT3: Mechanisms and Therapeutic Implications. [Abstract]2026 Apr 24;15(5):543. PMID: 42193166 -
Antioxidants (Basel)
3,6'-Disinapoyl Sucrose from Polygalae Radix Exerts Anti-Aging Effects via Modification of Telomeres, SIRT1/p53/p21 Pathway, Oxidative Stress and Autophagy. [Abstract]2026 Mar 1;15(3):313. PMID: 41897459 -
Antioxidants (Basel)
Arctigenin from Fructus arctii Exhibits Antiaging Effects via Autophagy Induction, Antioxidative Stress, and Increase in Telomerase Activity in Yeast. [Abstract]2024 Jun 2;13(6):684. PMID: 38929123 -
Phytother Res
Oridonin Ameliorates Alzheimer's Disease-Like Pathology in Male Mice Through Inhibition of Receptor-Interacting Protein Kinase 1. [Abstract]2026 May;40(5):2270-2287. PMID: 41677117 -
Free Radic Biol Med
Modulating hepatic hypoxanthine metabolism relieve metabolic stress-related neurovascular resilience disturbance via the liver-brain axis. [Abstract]2026 Jul 15:255:160-178. PMID: 42456994 -
Am J Chin Med
Cepharanthine Triggers Ferroptosis in Gastric Cancer by PINK1/FUNDC1-Mediated Mitophagy-Dependent GPX4 Degradation. [Abstract]2026;54(1):329-348. PMID: 41684093 -
Br J Pharmacol
Clemastine attenuates subarachnoid haemorrhage pathology in a mouse model via Nrf2/SQSTM1-mediated autophagy. [Abstract]2025 Jun;182(12):2730-2753. PMID: 40052261 -
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J Med Chem
Target Ligand Separation and Identification of Isoforsythiaside as a Histone Lysine-Specific Demethylase 1 Covalent Inhibitor Against Breast Cancer Metastasis. [Abstract]2024 Nov 14;67(21):19874-19888. PMID: 39499621 -
J Ethnopharmacol
Shuxuetong injection inhibits pyroptosis in acute ischemic stroke via CD44/NLRP3/GSDMD signal. [Abstract]2025 Apr 9:345:119618. PMID: 40074097 -
J Ethnopharmacol
Naoxintong capsule accelerates mitophagy in cerebral ischemia-reperfusion injury via TP53/PINK1/PRKN pathway based on network pharmacology analysis and experimental validation. [Abstract]2025 Jan 10:336:118721. PMID: 39173723 -
J Agric Food Chem
Acacetin Alleviates DSS-Induced Ulcerative Colitis by Targeting Keap1/Nrf2 Pathway to Inhibit Endoplasmic Reticulum Stress-Mediated Apoptosis. [Abstract]2026 Feb 11;74(5):4420-4434. PMID: 41612915 -
J Agric Food Chem
Efficient Discovery of Syk-Targeting Phenolic Acids from Orthosiphon aristatus and Their Antiplatelet Activities. [Abstract]2025 Dec 25. PMID: 41449956 -
J Agric Food Chem
Glabridin from Glycyrrhiza glabra L. Exhibits Anti-Triple-Negative Breast Cancer Effects by Regulating Various Cell Cycle Genes. [Abstract]2025 Sep 24;73(38):24055-24075. PMID: 40955780 -
CNS Neurosci Ther
Network Pharmacology-Based and Experimental Validation Elucidate the Target Mechanism of Vinorine in Ameliorating Secondary Brain Injury After Intracerebral Hemorrhage. [Abstract]2025 Sep;31(9):e70609. PMID: 40994248 -
Biochem Pharmacol
Acacetin targets SNX5 to promote autophagy degradation of NLRP3 inflammasome against cognitive impairment in Alzheimer's disease. [Abstract]2026 Sep;251(Pt 1):118103. PMID: 42178051 -
Biochem Pharmacol
2026 Jun 20;251(Pt 2):118181. PMID: 42323025 -
Biochem Pharmacol
Mairin polarizes Macrophages into M2-phenotype and alleviates Ulcerative colitis through activating IRF4-CD5L pathway. [Abstract]2026 May:247:117771. PMID: 41651133 -
Biochem Pharmacol
Tamibarotene directly targets the NACHT domain of NLRP3 to alleviate acute myocardial infarction. [Abstract]2025 Apr:234:116801. PMID: 39952330 -
Life Sci
2025 Jun 1:370:123568. PMID: 40113075 -
Life Sci
Nitidine chloride inhibits mTORC1 signaling through ATF4-mediated Sestrin2 induction and targets IGF2R for lysosomal degradation. [Abstract]2024 Sep 15:353:122918. PMID: 39034027 -
Int J Pharm
Functional doxorubicin and iron ions dual-loaded carboxymethyl chitin-based microspheres for hepatocellular carcinoma therapy via embolization, ferroptosis, and autophagy-mediated HIF-1α inhibition. [Abstract]2026 Mar 10:692:126628. PMID: 41616983 -
J Gastroenterol
Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis. [Abstract]2024 Nov;59(11):1037-1051. PMID: 39145797 -
Eur J Pharmacol
Arbidol attenuates liver fibrosis and activation of hepatic stellate cells by blocking TGF-β1 signaling. [Abstract]2024 Mar 15:967:176367. PMID: 38325795 -
Eur J Pharmacol
The neuroprotective effect of dl-3-n-butylphthalide on the brain with experimental intracerebral hemorrhage. [Abstract]2023 Nov 15:959:176105. PMID: 37802280 -
Int Immunopharmacol
Targeting Grx1 by hyperoside regulates Ncf4 post-translational modification to enhance mitophagy and accelerate diabetic wound healing. [Abstract]2026 Jun 26:186:117074. PMID: 42361530 -
Int J Mol Sci
Myrtenol from Lavender Essential Oil Possesses Neuroprotective Effects and Promotes Neurite Outgrowth by Potentially Targeting TrkA and IGF-1R in PC12 Cells. [Abstract]2026 Mar 12;27(6):2615. PMID: 41898478 -
Int Immunopharmacol
Ponicidin ameliorates Alzheimer's disease through dual inhibition of RIPK1-mediated neuroinflammation and necroptosis. [Abstract]2026 Feb 15:171:116095. PMID: 41477991 -
Int J Mol Sci
IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells. [Abstract]2024 Sep 10;25(18):9763. PMID: 39337253 -
Int Immunopharmacol
6-Gingerol ameliorates alveolar hypercoagulation and fibrinolytic inhibition in LPS-provoked ARDS via RUNX1/NF-κB signaling pathway. [Abstract]2024 Feb 15:128:111459. PMID: 38181675 -
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ACS Omega
2026 Feb 19;11(8):13768-13788. PMID: 41799095 -
Chem Biol Interact
HTR1D regulates the PI3K/Akt signaling pathway to impact glioblastoma development and resistance to temozolomide. [Abstract]2025 Nov 1:421:111748. PMID: 40972861 -
Chem Biol Interact
Liquiritigenin regulates MAPK (p38/JNK) signaling through inhibition of IRAK4, attenuates inflammatory response, fibrosis and kidney dysfunction in a high-salt diet induced chronic kidney disease. [Abstract]2025 Sep 5:418:111578. PMID: 40447172 -
Int J Antimicrob Agents
Therapeutic Effect of Taxifolin on Bacterial Meningitis Associated with Inhibition of PI3K/AKT and MAPK Signaling. [Abstract]2025 Aug 23:107601. PMID: 40854451 -
Sci Rep
Peptidoglycan-reshuffling proteins SCO0954, SCO1758, SCO4439, and SCO4440 modulate the formation of wall-deficient cells in Streptomyces coelicolor under hyperosmotic sucrose stress. [Abstract]2025 Sep 1;15(1):32112. PMID: 40890197 -
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FASEB J
Phellopterin Inhibits Inflammation and Relieves Cartilage Degradation by Suppressing MAPK Signaling in Osteoarthritis. [Abstract]2026 Mar 31;40(6):e71698. PMID: 41870411 -
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ACS Chem Biol
Key Amino Acid Residues of Mitochondrial Transcription Factor A Synergize with Abasic (AP) Site Dynamics To Facilitate AP-Lyase Reactions. [Abstract]2023 May 19;18(5):1168-1179. PMID: 36930463 -
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Dig Dis Sci
Dihydroartemisinin: A Targeting Agent of CCT3-Mediated Oxidative Stress to Enhance the Antitumor Activity of Doxorubicin in Hepatocellular Carcinoma. [Abstract]2025 Nov 8. PMID: 41205108 -
Toxicon
A Dendrobium chrysotoxum extract erianin induce AML cells death by activating PPARɑ and downregulating PI3K/AKT signaling pathways. [Abstract]2025 Jul:261:108371. PMID: 40280444 -
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Solvent & Solubility
In Vitro:
H2O : 10 mg/mL (Need ultrasonic)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)