Pronase E (Activity ≥ 4000 U/mg)
Based on 27 publication(s) in Google Scholar
Pronase E (Activity ≥ 4000 U/mg) is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus and could digest protein into individual amino acids.
For research use only. We do not sell to patients.
- CAS No.: 9036-06-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Pronase E (Activity ≥ 4000 U/mg)
More- Cell. 2026 Mar 5;189(5):1451-1464.e27. [Abstract]
- Cancer Res. 2026 Jul 15;86(14):3436-3458. [Abstract]
- Acta Pharm Sin B. 2025 Mar;15(3):1514-1534. [Abstract]
- Pharmacol Res. 2024 Apr:202:107128. [Abstract]
- Phytomedicine. 2026 Jul:156:158180. [Abstract]
- Phytomedicine. 2026 Jul:156:158202. [Abstract]
- Phytomedicine. 2026 Jun:155:158086. [Abstract]
- Phytomedicine. 2025 Dec:149:157524. [Abstract]
- Phytomedicine. 2025 Sep:145:156915. [Abstract]
- Phytomedicine. 2024 Dec:135:156011. [Abstract]
- J Pharm Anal. 2025 Dec;15(12):101307. [Abstract]
- Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. [Abstract]
- Arch Pharm Res. 2026 Mar;49(3):357-374. [Abstract]
- Free Radic Biol Med. 2025 May:232:353-366. [Abstract]
- Chin Med. 2022 Sep 29;17(1):115. [Abstract]
- Biochem Pharmacol. 2025 Nov:241:117158. [Abstract]
- Biochem Pharmacol. 2025 Sep 21;242(Pt 3):117360. [Abstract]
- Food Funct. 2026 Jun 22;17(12):5378-5393. [Abstract]
- Eur J Pharmacol. 2025 Nov 15:1007:178274. [Abstract]
- Int Immunopharmacol. 2026 May 1:176:116462. [Abstract]
- Int Immunopharmacol. 2026 Mar 15:173:116255. [Abstract]
- Am J Respir Cell Mol Biol. 2026 Jan 23:aanaf010. [Abstract]
- Bioorg Chem. 2025 Sep 17:165:109010. [Abstract]
- Virus Res. 2025 May:355:199561. [Abstract]
- G3 (Bethesda). 2022 May 6;12(5):jkac085. [Abstract]
- Res Sq. 2026 Jun 12.
- SSRN. 2025 Feb 4.
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Biological Activity
Pronase E significantly increases the amount of most of the amino acids analysed (PE vs C) , especially Ile, His and Thr[1]. Pronase E Usage Steps:. 1. Preparation of Stock Solution: (1) Dissolve Pronase E in deionized water to prepare a stock solution at a concentration of 5-20 mg/ml. Note: If used for DNA or RNA isolation steps, it is recommended to first heat the solution at 56°C for 15 minutes. (2) Incubate at 37°C for 1 hour. Aliquot the prepared stock solution into single-use samples and store at -20°C; typically stable for one year. 2. DNA Isolation: Directly add Pronase E to the DNA preparation system. The system should contain 0.5-1% SDS to disrupt DNA-protein interactions. Note: Use at a concentration of 250-500 μg protein/ml. Incubation conditions: Incubate at 37°C for 1-4 hours. 3. Protein Digestion: (1) Dissolve approximately 0.2 μM protein in 0.2 ml of 50 mM ammonium bicarbonate buffer (pH 8.0) or phosphate buffer (pH 7.0). (2) Add 1% (w/w) Pronase E. Incubate at 37°C for 24 hours to perform protein digestion.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9036-06-0
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Pronase E (Activity ≥ 4000 U/mg)]
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Synonyms
Pronase (Activity ≥ 4000 U/mg)
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Structure Classification
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Initial Source
Streptomyces griseus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (27)
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Journal Impact Factor
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Most Recent
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Cell
Structural decoding of reversible covalent linkage of odorants in human olfactory receptor OR6A2. [Abstract]2026 Mar 5;189(5):1451-1464.e27. PMID: 41570822 -
Cancer Res
TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma. [Abstract]2026 Jul 15;86(14):3436-3458. PMID: 41974002 -
Acta Pharm Sin B
Dihydromyricetin mitigates abdominal aortic aneurysm via transcriptional and post-transcriptional regulation of heme oxygenase-1 in vascular smooth muscle cells. [Abstract]2025 Mar;15(3):1514-1534. PMID: 40370559
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Mar;15(3):1514-1534. [Abstract]
The cell lysis was treated with DHM at concentrations of 0, 5, and 20 mmol/L, and then underwent Pronase E (20 mg/mL, 20 min) digestion.
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Pharmacol Res
Vanillic acid restores homeostasis of intestinal epithelium in colitis through inhibiting CA9/STIM1-mediated ferroptosis. [Abstract]2024 Apr:202:107128. PMID: 38438089
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Apr:202:107128. [Abstract]
Cell lysis was incubated with VA (0, 12.5, 25, and 50 μM) and then subjected to Pronase E (20 μg/mL, 30 min) digestion.
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Phytomedicine
Daphnoretin targeted binding to HSP90AA1 to promote P53 UFMylation and stability thereby inducing apoptosis in colorectal cancer. [Abstract]2026 Jul:156:158180. PMID: 42034025 -
Phytomedicine
Tubeimoside Ⅰ targets sodium/potassium ATPase alpha 1 subunit to selectively eliminate senescent cells and alleviate aging-associated abnormalities. [Abstract]2026 Jul:156:158202. PMID: 42035526 -
Phytomedicine
Usenamine a potentiates anti-CRC activity of sorafenib by inducing autophagy and inhibiting YAP pathway through targeting SOD2. [Abstract]2026 Jun:155:158086. PMID: 41861687 -
Phytomedicine
Icariin rescues immune dysfunction in sepsis through RSK2-driven YAP-cGAS-IFN-β signaling activation. [Abstract]2025 Dec:149:157524. PMID: 41240541
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157524. [Abstract]
Pronase E (20 μg/mL, 30 min). The experimental results of DARST and quantification graphs.
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Phytomedicine
Emodin-induced ERα degradation via SYVN1 alleviates vascular calcification by preventing HIF-1α deacetylation in chronic kidney disease. [Abstract]2025 Sep:145:156915. PMID: 40541127
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:156915. [Abstract]
T/G-HA-VSMC lysates were incubated with Emodin (0, 5, 10, and 20 μM) and then subjected to Pronase E (40 ng/mL, 15 min) digestion. The protein levels of SYVN1 were measured by Western Blot.
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Phytomedicine
Corilagin alleviated intestinal ischemia-reperfusion injury by modulating endoplasmic reticulum stress via bonding with Bip. [Abstract]2024 Dec:135:156011. PMID: 39265205 -
J Pharm Anal
1,8-Cineole ameliorates vascular endothelial senescence in diabetes mellitus by directly targeting and deubiquitinating PPAR-γ in vivo and in vitro. [Abstract]2025 Dec;15(12):101307. PMID: 41492461
Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec;15(12):101307. [Abstract]
Western blotting results of the DARTS experiment indicating that the PPAR-g protein was more tolerant to Pronase E (10 mg/mL, 10 min) after 1,8-cineole treatment.
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Cell Chem Biol
2022 Mar 17;29(3):373-385.e6. PMID: 34706270 -
Arch Pharm Res
Homoharringtonine inhibits growth and migration in non-small cell lung cancer via PDIA4-mediated modulation of autophagy and EMT. [Abstract]2026 Mar;49(3):357-374. PMID: 41826608 -
Free Radic Biol Med
Kirenol alleviates cerebral ischemia-reperfusion injury by reducing oxidative stress and ameliorating mitochondrial dysfunction via activating the CK2/AKT pathway. [Abstract]2025 May:232:353-366. PMID: 40090600 -
Chin Med
Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. [Abstract]2022 Sep 29;17(1):115. PMID: 36175965 -
Biochem Pharmacol
Promoting ubiquitin-dependent Drp1 degradation contributes to the protective effect of Astragalin against diabetic renal fibrosis. [Abstract]2025 Nov:241:117158. PMID: 40659133 -
Biochem Pharmacol
Chemoproteomics-based profiling reveals the targeting molecular mechanism of reversal of cisplatin resistance by baicalin in head and neck squamous cell carcinoma. [Abstract]2025 Sep 21;242(Pt 3):117360. PMID: 40987408 -
Food Funct
Cynarin alleviates apical periodontitis by inhibiting macrophage M1 polarization via the PGC1α/ROS signaling axis. [Abstract]2026 Jun 22;17(12):5378-5393. PMID: 42132631 -
Eur J Pharmacol
Neoprzewaquinone A suppresses hepatocellular carcinoma through promoting the ubiquitin-related degradation of EGFR and inhibiting PI3K-AKT pathway. [Abstract]2025 Nov 15:1007:178274. PMID: 41130376 -
Int Immunopharmacol
2026 May 1:176:116462. PMID: 41797086 -
Int Immunopharmacol
Neogambogic acid promotes M-MDSC differentiation into M1 macrophages to inhibit peritoneal metastasis of colorectal cancer. [Abstract]2026 Mar 15:173:116255. PMID: 41579798 -
Am J Respir Cell Mol Biol
Lactylation-YTHDF1 Axis Promotes DNAH5-dependent Ciliary Defense in Pseudomonas aeruginosa Infection. [Abstract]2026 Jan 23:aanaf010. PMID: 41738162 -
Bioorg Chem
Usenamine A, an RGS2 inhibitor, exerts anti-NSCLC activity and enhances cytotoxicity of gemcitabine by inducing ER stress and Notch1-mediated autophagy. [Abstract]2025 Sep 17:165:109010. PMID: 40974657 -
Virus Res
Regulation of Inflammatory Cytokines and Activation of PI3K/Akt Pathway by Yiqi Jiedu Formula in HSV-1-Induced Keratitis: Experimental and Network Pharmacology Evidence. [Abstract]2025 May:355:199561. PMID: 40120648 -
G3 (Bethesda)
Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. [Abstract]2022 May 6;12(5):jkac085. PMID: 35404452 -
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Solvent & Solubility
H2O : ≥ 66.67 mg/mL
* "≥" means soluble, but saturation unknown.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL; Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (272 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)