Art. -Nr.: HY-114158A

Pronase E (Activity ≥ 4000 U/mg)

Name: Pronase E (Activity ≥ 4000 U/mg)
Brand: MedChemExpress
SKU: HY-114158A
Price: 60.00 USD
Availability: InStock
Rating: 5 (32 reviews)

(Synonyms: Pronase (Activity ≥ 4000 U/mg))

32 Cited Publications

Kundenbewertung

Based on 32 publication(s) in Google Scholar

Pronase E (Activity ≥ 4000 U/mg) is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus and could digest protein into individual amino acids.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 9036-06-0

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Pronase E (Activity ≥ 4000 U/mg) Chemical Structure

CAS. Nr.: 9036-06-0

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (32)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Pronase E (Activity ≥ 4000 U/mg) is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus and could digest protein into individual amino acids^[1].

In Vitro

Pronase E significantly increases the amount of most of the amino acids analysed (PE vs C) , especially Ile, His and Thr^[1].

Pronase E Usage Steps:.
1. Preparation of Stock Solution:
(1) Dissolve Pronase E in deionized water to prepare a stock solution at a concentration of 5-20 mg/ml.
Note: If used for DNA or RNA isolation steps, it is recommended to first heat the solution at 56°C for 15 minutes.
(2) Incubate at 37°C for 1 hour. Aliquot the prepared stock solution into single-use samples and store at -20°C; typically stable for one year.
2. DNA Isolation:
Directly add Pronase E to the DNA preparation system. The system should contain 0.5-1% SDS to disrupt DNA-protein interactions.
Note: Use at a concentration of 250-500 μg protein/ml. Incubation conditions: Incubate at 37°C for 1-4 hours.
3. Protein Digestion:
(1) Dissolve approximately 0.2 μM protein in 0.2 ml of 50 mM ammonium bicarbonate buffer (pH 8.0) or phosphate buffer (pH 7.0).
(2) Add 1% (w/w) Pronase E. Incubate at 37°C for 24 hours to perform protein digestion.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Experimental Procedure for Isolating Primary Hematopoietic Stem Cells (HSCs) from Mice Using Pronase E^[2]
1. Animal Anesthesia and Abdominal Exposure:
(1) Anesthetize male or female mice.
(2) Under sterile conditions, expose the liver via abdominal incision.
2. Liver Perfusion Procedure:
(1) Perform liver perfusion through the portal vein using Hanks' Balanced Salt Solution (HBSS) to remove blood and impurities.
(2) Conduct enzymatic perfusion with Pronase E and collagenase to dissociate liver tissue.
3. Liver Extraction and Cell Release:
(1) Carefully extract the liver from the abdominal cavity using sterile forceps, and quickly transfer it to a sterile culture dish.
(2) Gently peel the liver capsule and apply mild mechanical agitation to release cells from the liver tissue.
4. Cell Digestion and Preliminary Centrifugation:
(1) Suspend the released cells in a mixed enzyme solution containing Pronase E, collagenase, and DNase, and digest at 37°C for 20 minutes.
(2) Perform two rounds of low-speed centrifugation (50 g, 3 minutes) to separate liver cells, and collect the cell suspension.
5. HSC Separation and Density Gradient Centrifugation:
(1) Further centrifuge the cell suspension (450 g, 8 minutes) and collect the pellet.
(2) Resuspend the pellet in 18% Nycodenz solution as the basis for density gradient separation.
(3) Construct a density gradient with 12% Nycodenz, 8.2% Nycodenz, and Gey's Balanced Salt Solution (GBSS).
(4) Isolate and purify HSCs from the 8.2% Nycodenz layer through density gradient centrifugation.
Note: This protocol provides standard operating procedures; please adjust and optimize according to specific experimental needs and conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 9036-06-0
Appearance Solid
Color Off-white to light yellow
SMILES

[Pronase E (Activity ≥ 4000 U/mg)]
Synonyms

Pronase (Activity ≥ 4000 U/mg)
Initial Source
- Microorganism
Streptomyces griseus
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (32)

All WB

Sort by:

Journal Impact Factor

Cell

Structural decoding of reversible covalent linkage of odorants in human olfactory receptor OR6A2. [Abstract]

2026 Mar 5;189(5):1451-1464.e27. PMID: 41570822
Cancer Res

TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma. [Abstract]

2026 Jul 15;86(14):3436-3458. PMID: 41974002
Acta Pharm Sin B

Dihydromyricetin mitigates abdominal aortic aneurysm via transcriptional and post-transcriptional regulation of heme oxygenase-1 in vascular smooth muscle cells. [Abstract]

2025 Mar;15(3):1514-1534. PMID: 40370559

Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Mar;15(3):1514-1534. [Abstract]

The cell lysis was treated with DHM at concentrations of 0, 5, and 20 mmol/L, and then underwent Pronase E (20 mg/mL, 20 min) digestion.
Cell Death Dis

2026 Aug 31.
Pharmacol Res

Vanillic acid restores homeostasis of intestinal epithelium in colitis through inhibiting CA9/STIM1-mediated ferroptosis. [Abstract]

2024 Apr:202:107128. PMID: 38438089

Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Apr:202:107128. [Abstract]

Cell lysis was incubated with VA (0, 12.5, 25, and 50 μM) and then subjected to Pronase E (20 μg/mL, 30 min) digestion.
Phytomedicine

Tubeimoside Ⅰ targets sodium/potassium ATPase alpha 1 subunit to selectively eliminate senescent cells and alleviate aging-associated abnormalities. [Abstract]

2026 Jul:156:158202. PMID: 42035526
Phytomedicine

Daphnoretin targeted binding to HSP90AA1 to promote P53 UFMylation and stability thereby inducing apoptosis in colorectal cancer. [Abstract]

2026 Jul:156:158180. PMID: 42034025
Phytomedicine

Usenamine a potentiates anti-CRC activity of sorafenib by inducing autophagy and inhibiting YAP pathway through targeting SOD2. [Abstract]

2026 Jun:155:158086. PMID: 41861687
Phytomedicine

Icariin rescues immune dysfunction in sepsis through RSK2-driven YAP-cGAS-IFN-β signaling activation. [Abstract]

2025 Dec:149:157524. PMID: 41240541

Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157524. [Abstract]

Pronase E (20 μg/mL, 30 min). The experimental results of DARST and quantification graphs.
Phytomedicine

Emodin-induced ERα degradation via SYVN1 alleviates vascular calcification by preventing HIF-1α deacetylation in chronic kidney disease. [Abstract]

2025 Sep:145:156915. PMID: 40541127

Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:156915. [Abstract]

T/G-HA-VSMC lysates were incubated with Emodin (0, 5, 10, and 20 μM) and then subjected to Pronase E (40 ng/mL, 15 min) digestion. The protein levels of SYVN1 were measured by Western Blot.
Phytomedicine

Corilagin alleviated intestinal ischemia-reperfusion injury by modulating endoplasmic reticulum stress via bonding with Bip. [Abstract]

2024 Dec:135:156011. PMID: 39265205
J Pharm Anal

1,8-Cineole ameliorates vascular endothelial senescence in diabetes mellitus by directly targeting and deubiquitinating PPAR-γ in vivo and in vitro. [Abstract]

2025 Dec;15(12):101307. PMID: 41492461

Pronase E (Activity ≥ 4000 U/mg) purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec;15(12):101307. [Abstract]

Western blotting results of the DARTS experiment indicating that the PPAR-g protein was more tolerant to Pronase E (10 mg/mL, 10 min) after 1,8-cineole treatment.
Adv Healthc Mater

2026 Sep;15(36):e71628. PMID: 42629983
Cell Chem Biol

2026 Sep 15:S2451-9456(26)00322-3. PMID: 42743924
Cell Chem Biol

Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. [Abstract]

2022 Mar 17;29(3):373-385.e6. PMID: 34706270
Arch Pharm Res

Homoharringtonine inhibits growth and migration in non-small cell lung cancer via PDIA4-mediated modulation of autophagy and EMT. [Abstract]

2026 Mar;49(3):357-374. PMID: 41826608
Free Radic Biol Med

Kirenol alleviates cerebral ischemia-reperfusion injury by reducing oxidative stress and ameliorating mitochondrial dysfunction via activating the CK2/AKT pathway. [Abstract]

2025 May:232:353-366. PMID: 40090600
Transl Psychiatry

2026 Aug 19.
Chin Med

Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. [Abstract]

2022 Sep 29;17(1):115. PMID: 36175965
Biochem Pharmacol

2026 Sep 9;254(Pt 2):118462. PMID: 42716134
Biochem Pharmacol

Promoting ubiquitin-dependent Drp1 degradation contributes to the protective effect of Astragalin against diabetic renal fibrosis. [Abstract]

2025 Nov:241:117158. PMID: 40659133
Biochem Pharmacol

Chemoproteomics-based profiling reveals the targeting molecular mechanism of reversal of cisplatin resistance by baicalin in head and neck squamous cell carcinoma. [Abstract]

2025 Sep 21;242(Pt 3):117360. PMID: 40987408
Food Funct

Cynarin alleviates apical periodontitis by inhibiting macrophage M1 polarization via the PGC1α/ROS signaling axis. [Abstract]

2026 Jun 22;17(12):5378-5393. PMID: 42132631
Eur J Pharmacol

Neoprzewaquinone A suppresses hepatocellular carcinoma through promoting the ubiquitin-related degradation of EGFR and inhibiting PI3K-AKT pathway. [Abstract]

2025 Nov 15:1007:178274. PMID: 41130376
Int Immunopharmacol

Α-Hederin alleviates psoriasiform skin inflammation by inhibiting NF-κB p65/CXCL2 Axis. [Abstract]

2026 May 1:176:116462. PMID: 41797086
Int Immunopharmacol

Neogambogic acid promotes M-MDSC differentiation into M1 macrophages to inhibit peritoneal metastasis of colorectal cancer. [Abstract]

2026 Mar 15:173:116255. PMID: 41579798
Am J Respir Cell Mol Biol

Lactylation-YTHDF1 Axis Promotes DNAH5-dependent Ciliary Defense in Pseudomonas aeruginosa Infection. [Abstract]

2026 Jan 23:aanaf010. PMID: 41738162
Bioorg Chem

Usenamine A, an RGS2 inhibitor, exerts anti-NSCLC activity and enhances cytotoxicity of gemcitabine by inducing ER stress and Notch1-mediated autophagy. [Abstract]

2025 Sep 17:165:109010. PMID: 40974657
Virus Res

Regulation of Inflammatory Cytokines and Activation of PI3K/Akt Pathway by Yiqi Jiedu Formula in HSV-1-Induced Keratitis: Experimental and Network Pharmacology Evidence. [Abstract]

2025 May:355:199561. PMID: 40120648
G3 (Bethesda)

Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. [Abstract]

2022 May 6;12(5):jkac085. PMID: 35404452
Res Sq

2026 Jun 12.
SSRN

2025 Feb 4.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 66.67 mg/mL

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. José M Bruna, et al. Enhancement of the flavour development of dry fermented sausages by using a protease (Pronase E) and a cell‐free extract of Penicillium camemberti. Journal of the science of food and agriculture. Volume82, Issue5. April 2002. Pages 526-533.

[2]. Chen QT, et al. HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma. Nat Metab. 2022 Oct;4(10):1306-1321. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

Pronase E (Activity ≥ 4000 U/mg)

32 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

Pronase E (Activity ≥ 4000 U/mg)

Publications Citing Use of MedChemExpress (MCE) Pronase E (Activity ≥ 4000 U/mg)