Pronase E (Activity ≥ 7000 U/g)
Based on 72 publication(s) in Google Scholar
Pronase E (Activity ≥ 7000 U/g) is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus and could digest protein into individual amino acids.
For research use only. We do not sell to patients.
- CAS No.: 9036-06-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) Pronase E (Activity ≥ 7000 U/g)
More- Nat Metab. 2022 Oct;4(10):1306-1321. [Abstract]
- Autophagy. 2026 May 25:1-21. [Abstract]
- Autophagy. 2026 Mar;22(3):526-544. [Abstract]
- Autophagy. 2025 Jun;21(6):1316-1334. [Abstract]
- Autophagy. 2023 Aug;19(8):2240-2256. [Abstract]
- J Adv Res. 2025 Jul 14:S2090-1232(25)00544-2. [Abstract]
- Redox Biol. 2026 Mar:90:104055. [Abstract]
- Acta Pharm Sin B. 2026 Jul;16(7):4410-4425. [Abstract]
- Adv Sci (Weinh). 2026 Jun 19:e76165. [Abstract]
- Adv Sci (Weinh). 2026 Feb 28:e21818. [Abstract]
- Cell Rep Med. 2026 Jun 16;7(6):102826. [Abstract]
- Sci Adv. 2023 Jul 21;9(29):eadh4054. [Abstract]
- Pharmacol Res. 2024 Dec:210:107516. [Abstract]
- Cell Commun Signal. 2024 Oct 11;22(1):488. [Abstract]
- Phytomedicine. 2026 Jul:156:158224. [Abstract]
- Phytomedicine. 2026 Jun:155:158157. [Abstract]
- Phytomedicine. 2026 Jan:150:157584. [Abstract]
- Phytomedicine. 2025 Nov 25:148:157395. [Abstract]
- Phytomedicine. 2025 Oct:146:157126. [Abstract]
- Phytomedicine. 2025 Sep:145:156994. [Abstract]
- Phytomedicine. 2024 Dec:135:156139. [Abstract]
- Phytomedicine. 2024 Oct:133:155947. [Abstract]
- J Pharm Anal. 2025 Dec;15(12):101301. [Abstract]
- Antioxidants (Basel). 2026 Apr 24;15(5):543. [Abstract]
- Antioxidants (Basel). 2026 Mar 1;15(3):313. [Abstract]
- Antioxidants (Basel). 2024 Jun 2;13(6):684. [Abstract]
- Phytother Res. 2026 May;40(5):2270-2287. [Abstract]
- Am J Chin Med. 2026;54(1):329-348. [Abstract]
- Br J Pharmacol. 2025 Jun;182(12):2730-2753. [Abstract]
- J Med Chem. 2024 Nov 14;67(21):19874-19888. [Abstract]
- J Ethnopharmacol. 2025 Apr 9:345:119618. [Abstract]
- J Ethnopharmacol. 2025 Jan 10:336:118721. [Abstract]
- J Agric Food Chem. 2026 Feb 11;74(5):4420-4434. [Abstract]
- J Agric Food Chem. 2025 Dec 25. [Abstract]
- J Agric Food Chem. 2025 Sep 24;73(38):24055-24075. [Abstract]
- CNS Neurosci Ther. 2025 Sep;31(9):e70609. [Abstract]
- Biochem Pharmacol. 2026 Sep;251(Pt 1):118103. [Abstract]
- Biochem Pharmacol. 2026 Jun 20;251(Pt 2):118181. [Abstract]
- Biochem Pharmacol. 2026 May:247:117771. [Abstract]
- Biochem Pharmacol. 2025 Apr:234:116801. [Abstract]
- Life Sci. 2025 Jun 1:370:123568. [Abstract]
- Life Sci. 2024 Sep 15:353:122918. [Abstract]
- Int J Pharm. 2026 Mar 10:692:126628. [Abstract]
- J Gastroenterol. 2024 Nov;59(11):1037-1051. [Abstract]
- Eur J Pharmacol. 2024 Mar 15:967:176367. [Abstract]
- Eur J Pharmacol. 2023 Nov 15:959:176105. [Abstract]
- Int Immunopharmacol. 2026 Jun 26:186:117074. [Abstract]
- Int J Mol Sci. 2026 Mar 12;27(6):2615. [Abstract]
- Int Immunopharmacol. 2026 Feb 15:171:116095. [Abstract]
- Int J Mol Sci. 2024 Sep 10;25(18):9763. [Abstract]
- Int Immunopharmacol. 2024 Feb 15:128:111459. [Abstract]
- ACS Omega. 2026 Jun 17.
- Microchem J. 2026 Mar 31;224:117898.
- ACS Omega. 2026 Feb 19;11(8):13768-13788. [Abstract]
- Chem Biol Interact. 2025 Nov 1:421:111748. [Abstract]
- Chem Biol Interact. 2025 Sep 5:418:111578. [Abstract]
- Int J Antimicrob Agents. 2025 Aug 23:107601. [Abstract]
- Sci Rep. 2025 Sep 1;15(1):32112. [Abstract]
- Aquaculture. 2025 May 1.
- FASEB J. 2026 Mar 31;40(6):e71698. [Abstract]
- Food Biophys. 2025 Aug 6.
- ACS Chem Biol. 2023 May 19;18(5):1168-1179. [Abstract]
- Dig Dis Sci. 2025 Nov 8. [Abstract]
- Toxicon. 2025 Jul:261:108371. [Abstract]
- SSRN. 2025 Oct 9.
- University of Alabama. 2025.
- University of California, Riverside. 2024.
- bioRxiv. 2024 September 08.
- University of East Anglia. 2024 Apr.
- SSRN. 2023 Jul 31.
- SSRN. 2023 Mar 13.
- Research Square Print. 2022 Aug.
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Biological Activity
Pronase E significantly increases the amount of most of the amino acids analysed (PE vs C) , especially Ile, His and Thr[1]. Pronase E Usage Steps:. 1. Preparation of Stock Solution: (1) Dissolve Pronase E in deionized water to prepare a stock solution at a concentration of 5-20 mg/ml. Note: If used for DNA or RNA isolation steps, it is recommended to first heat the solution at 56°C for 15 minutes. (2) Incubate at 37°C for 1 hour. Aliquot the prepared stock solution into single-use samples and store at -20°C; typically stable for one year. 2. DNA Isolation: Directly add Pronase E to the DNA preparation system. The system should contain 0.5-1% SDS to disrupt DNA-protein interactions. Note: Use at a concentration of 250-500 μg protein/ml. Incubation conditions: Incubate at 37°C for 1-4 hours. 3. Protein Digestion: (1) Dissolve approximately 0.2 μM protein in 0.2 ml of 50 mM ammonium bicarbonate buffer (pH 8.0) or phosphate buffer (pH 7.0). (2) Add 1% (w/w) Pronase E. Incubate at 37°C for 24 hours to perform protein digestion.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 9036-06-0
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Pronase E (Activity?7000 U/g)]
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Synonyms
Pronase (Activity ≥ 7000 U/g)
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Structure Classification
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Initial Source
Streptomyces griseus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (72)
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Journal Impact Factor
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Most Recent
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Nat Metab
HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma. [Abstract]2022 Oct;4(10):1306-1321. PMID: 36192599 -
Autophagy
Byakangelicin alleviates metabolic dysfunction-associated steatohepatitis by selective inhibition of a non-canonical MTORC1 signaling pathway. [Abstract]2026 May 25:1-21. PMID: 42152469 -
Autophagy
Lactucopicrin promotes the autophagic degradation of MAP2K4/MKK4 by mediating CCDC50 palmitoylation to alleviate osteoarthritis progression. [Abstract]2026 Mar;22(3):526-544. PMID: 41566717 -
Autophagy
Buddleoside alleviates nonalcoholic steatohepatitis by targeting the AMPK-TFEB signaling pathway. [Abstract]2025 Jun;21(6):1316-1334. PMID: 39936600 -
Autophagy
Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin. [Abstract]2023 Aug;19(8):2240-2256. PMID: 36779633 -
J Adv Res
Tussilagone attenuated cigarette smoke-induced chronic obstructive pulmonary disease through regulating Nrf2 and NF-κB/NLRP3 inflammasome via directly targeting cysteine 434 of KEAP1. [Abstract]2025 Jul 14:S2090-1232(25)00544-2. PMID: 40669599 -
Redox Biol
DMNQ induces ferroptosis and augments the efficacy of anti-PD-L1 immunotherapy in gastric cancer via the STAT3/SLC1A4 axis to mediate cysteine metabolism reprogramming. [Abstract]2026 Mar:90:104055. PMID: 41604939 -
Acta Pharm Sin B
Insights into SARS-CoV-2 replication control via targeting the host hijacking function of NSP12. [Abstract]2026 Jul;16(7):4410-4425. PMID: 42453420 -
Adv Sci (Weinh)
Targeting the HSPA8-CMA-ATP6V1A Axis Triggers Lysosomal Hyperacidification and Catastrophic Vacuolation in Prostate Cancer. [Abstract]2026 Jun 19:e76165. PMID: 42318659 -
Adv Sci (Weinh)
Artesunate Ameliorates APAP-induced Liver Injury by Promoting NEDD4L-Mediated Ubiquitination and Degradation of TXNIP. [Abstract]2026 Feb 28:e21818. PMID: 41762705 -
Cell Rep Med
Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models. [Abstract]2026 Jun 16;7(6):102826. PMID: 42167246 -
Sci Adv
2023 Jul 21;9(29):eadh4054. PMID: 37478191 -
Pharmacol Res
Gentisic acid prevents the development of atherosclerotic lesions by inhibiting SNX10-mediated stabilization of LRP6. [Abstract]2024 Dec:210:107516. PMID: 39603572
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Dec:210:107516. [Abstract]
Cell lysis was incubated with GA (0, 12.5, 25, and 50 μM) and then subjected to Pronase E (20 μg/mL; 30 min) digestion. The protein level of SNX10 was measured by Western Blot and quantified by Image J software.
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Cell Commun Signal
Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling. [Abstract]2024 Oct 11;22(1):488. PMID: 39394127 -
Phytomedicine
Buyang Huanwu Decoction suppresses atherosclerosis by targeting IFITM1‑mediated lipid metabolism in vascular smooth muscle cells. [Abstract]2026 Jul:156:158224. PMID: 42030801 -
Phytomedicine
Schisantherin A alleviates DSS-induced colitis by upregulating ferredoxin 1 to inhibit inflammation and restore intestinal barrier function. [Abstract]2026 Jun:155:158157. PMID: 41966028 -
Phytomedicine
Licochalcone B targets neural cell adhesion molecule 1 to inhibit osteosarcoma progression and ferroptosis resistance via extracellular signal-regulated kinase 5 and nuclear factor kappa B pathways. [Abstract]2026 Jan:150:157584. PMID: 41349457
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jan:150:157584. [Abstract]
Pronase E (1:2000-1000; 30 min) and Licochalcone B (20 μM; 30 min). The NCAM1 protein expression of drug affinity responsive target stability assay (upper panel) and statistical analysis (lower panel). Ns, not significant. **, P <0.01; ***, P <0.001; ****, P <0.0001.
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Phytomedicine
Guben-Qushi-Huayu formula and calycosin improve epidermal barrier function in psoriasis by inhibiting small proline-rich protein 1A. [Abstract]2025 Nov 25:148:157395. PMID: 41166908 -
Phytomedicine
Dihydrocapsaicin attenuates oxidative stress and apoptosis in acute myocardial infarction via promoting Raf-1/ASK1 complex formation. [Abstract]2025 Oct:146:157126. PMID: 40768809 -
Phytomedicine
Tanshinone I alleviates post-ischemic myocardial injury by targeting TGFBR1 and modulating the TGF-β signaling pathway. [Abstract]2025 Sep:145:156994. PMID: 40580691
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:156994. [Abstract]
Effect of Tan I on TGFBR1 in Pronase E (500:1; 1h)-treated H9c2 cells.
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Phytomedicine
Shikonin induces ferroptosis in osteosarcomas through the mitochondrial ROS-regulated HIF-1α/HO-1 axis. [Abstract]2024 Dec:135:156139. PMID: 39423479 -
Phytomedicine
Silymarin targets the FXR protein through microbial metabolite 7-keto-deoxycholic acid to treat MASLD in obese mice. [Abstract]2024 Oct:133:155947. PMID: 39178642
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Oct:133:155947. [Abstract]
Pronase E (3 ug; 25 min) degrades FXR and β-actin by 1.5%. DARTS results of 7-KDCA on FXR.
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J Pharm Anal
α-hederin decreases the glycolysis level in intestinal epithelial cells via SNX10-mediated DEPDC5 degradation. [Abstract]2025 Dec;15(12):101301. PMID: 41487148
Pronase E (Activity ≥ 7000 U/g) purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec;15(12):101301. [Abstract]
Pronase E (25 μg/mL; 30 min). The binding of α-hed with SNX10 and DEPDC5 was determined by drug affinity responsive target stability (DARTS) assay.
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Antioxidants (Basel)
Empagliflozin Ameliorates Diabetic Cardiomyopathy by Inhibiting Ferroptosis via SIRT3: Mechanisms and Therapeutic Implications. [Abstract]2026 Apr 24;15(5):543. PMID: 42193166 -
Antioxidants (Basel)
3,6'-Disinapoyl Sucrose from Polygalae Radix Exerts Anti-Aging Effects via Modification of Telomeres, SIRT1/p53/p21 Pathway, Oxidative Stress and Autophagy. [Abstract]2026 Mar 1;15(3):313. PMID: 41897459 -
Antioxidants (Basel)
Arctigenin from Fructus arctii Exhibits Antiaging Effects via Autophagy Induction, Antioxidative Stress, and Increase in Telomerase Activity in Yeast. [Abstract]2024 Jun 2;13(6):684. PMID: 38929123 -
Phytother Res
Oridonin Ameliorates Alzheimer's Disease-Like Pathology in Male Mice Through Inhibition of Receptor-Interacting Protein Kinase 1. [Abstract]2026 May;40(5):2270-2287. PMID: 41677117 -
Am J Chin Med
Cepharanthine Triggers Ferroptosis in Gastric Cancer by PINK1/FUNDC1-Mediated Mitophagy-Dependent GPX4 Degradation. [Abstract]2026;54(1):329-348. PMID: 41684093 -
Br J Pharmacol
Clemastine attenuates subarachnoid haemorrhage pathology in a mouse model via Nrf2/SQSTM1-mediated autophagy. [Abstract]2025 Jun;182(12):2730-2753. PMID: 40052261 -
J Med Chem
Target Ligand Separation and Identification of Isoforsythiaside as a Histone Lysine-Specific Demethylase 1 Covalent Inhibitor Against Breast Cancer Metastasis. [Abstract]2024 Nov 14;67(21):19874-19888. PMID: 39499621 -
J Ethnopharmacol
Shuxuetong injection inhibits pyroptosis in acute ischemic stroke via CD44/NLRP3/GSDMD signal. [Abstract]2025 Apr 9:345:119618. PMID: 40074097 -
J Ethnopharmacol
Naoxintong capsule accelerates mitophagy in cerebral ischemia-reperfusion injury via TP53/PINK1/PRKN pathway based on network pharmacology analysis and experimental validation. [Abstract]2025 Jan 10:336:118721. PMID: 39173723 -
J Agric Food Chem
Acacetin Alleviates DSS-Induced Ulcerative Colitis by Targeting Keap1/Nrf2 Pathway to Inhibit Endoplasmic Reticulum Stress-Mediated Apoptosis. [Abstract]2026 Feb 11;74(5):4420-4434. PMID: 41612915 -
J Agric Food Chem
Efficient Discovery of Syk-Targeting Phenolic Acids from Orthosiphon aristatus and Their Antiplatelet Activities. [Abstract]2025 Dec 25. PMID: 41449956 -
J Agric Food Chem
Glabridin from Glycyrrhiza glabra L. Exhibits Anti-Triple-Negative Breast Cancer Effects by Regulating Various Cell Cycle Genes. [Abstract]2025 Sep 24;73(38):24055-24075. PMID: 40955780 -
CNS Neurosci Ther
Network Pharmacology-Based and Experimental Validation Elucidate the Target Mechanism of Vinorine in Ameliorating Secondary Brain Injury After Intracerebral Hemorrhage. [Abstract]2025 Sep;31(9):e70609. PMID: 40994248 -
Biochem Pharmacol
Acacetin targets SNX5 to promote autophagy degradation of NLRP3 inflammasome against cognitive impairment in Alzheimer's disease. [Abstract]2026 Sep;251(Pt 1):118103. PMID: 42178051 -
Biochem Pharmacol
2026 Jun 20;251(Pt 2):118181. PMID: 42323025 -
Biochem Pharmacol
Mairin polarizes Macrophages into M2-phenotype and alleviates Ulcerative colitis through activating IRF4-CD5L pathway. [Abstract]2026 May:247:117771. PMID: 41651133 -
Biochem Pharmacol
Tamibarotene directly targets the NACHT domain of NLRP3 to alleviate acute myocardial infarction. [Abstract]2025 Apr:234:116801. PMID: 39952330 -
Life Sci
2025 Jun 1:370:123568. PMID: 40113075 -
Life Sci
Nitidine chloride inhibits mTORC1 signaling through ATF4-mediated Sestrin2 induction and targets IGF2R for lysosomal degradation. [Abstract]2024 Sep 15:353:122918. PMID: 39034027 -
Int J Pharm
Functional doxorubicin and iron ions dual-loaded carboxymethyl chitin-based microspheres for hepatocellular carcinoma therapy via embolization, ferroptosis, and autophagy-mediated HIF-1α inhibition. [Abstract]2026 Mar 10:692:126628. PMID: 41616983 -
J Gastroenterol
Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis. [Abstract]2024 Nov;59(11):1037-1051. PMID: 39145797 -
Eur J Pharmacol
Arbidol attenuates liver fibrosis and activation of hepatic stellate cells by blocking TGF-β1 signaling. [Abstract]2024 Mar 15:967:176367. PMID: 38325795 -
Eur J Pharmacol
The neuroprotective effect of dl-3-n-butylphthalide on the brain with experimental intracerebral hemorrhage. [Abstract]2023 Nov 15:959:176105. PMID: 37802280 -
Int Immunopharmacol
Targeting Grx1 by hyperoside regulates Ncf4 post-translational modification to enhance mitophagy and accelerate diabetic wound healing. [Abstract]2026 Jun 26:186:117074. PMID: 42361530 -
Int J Mol Sci
Myrtenol from Lavender Essential Oil Possesses Neuroprotective Effects and Promotes Neurite Outgrowth by Potentially Targeting TrkA and IGF-1R in PC12 Cells. [Abstract]2026 Mar 12;27(6):2615. PMID: 41898478 -
Int Immunopharmacol
Ponicidin ameliorates Alzheimer's disease through dual inhibition of RIPK1-mediated neuroinflammation and necroptosis. [Abstract]2026 Feb 15:171:116095. PMID: 41477991 -
Int J Mol Sci
IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells. [Abstract]2024 Sep 10;25(18):9763. PMID: 39337253 -
Int Immunopharmacol
6-Gingerol ameliorates alveolar hypercoagulation and fibrinolytic inhibition in LPS-provoked ARDS via RUNX1/NF-κB signaling pathway. [Abstract]2024 Feb 15:128:111459. PMID: 38181675 -
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ACS Omega
2026 Feb 19;11(8):13768-13788. PMID: 41799095 -
Chem Biol Interact
HTR1D regulates the PI3K/Akt signaling pathway to impact glioblastoma development and resistance to temozolomide. [Abstract]2025 Nov 1:421:111748. PMID: 40972861 -
Chem Biol Interact
Liquiritigenin regulates MAPK (p38/JNK) signaling through inhibition of IRAK4, attenuates inflammatory response, fibrosis and kidney dysfunction in a high-salt diet induced chronic kidney disease. [Abstract]2025 Sep 5:418:111578. PMID: 40447172 -
Int J Antimicrob Agents
Therapeutic Effect of Taxifolin on Bacterial Meningitis Associated with Inhibition of PI3K/AKT and MAPK Signaling. [Abstract]2025 Aug 23:107601. PMID: 40854451 -
Sci Rep
Peptidoglycan-reshuffling proteins SCO0954, SCO1758, SCO4439, and SCO4440 modulate the formation of wall-deficient cells in Streptomyces coelicolor under hyperosmotic sucrose stress. [Abstract]2025 Sep 1;15(1):32112. PMID: 40890197 -
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FASEB J
Phellopterin Inhibits Inflammation and Relieves Cartilage Degradation by Suppressing MAPK Signaling in Osteoarthritis. [Abstract]2026 Mar 31;40(6):e71698. PMID: 41870411 -
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ACS Chem Biol
Key Amino Acid Residues of Mitochondrial Transcription Factor A Synergize with Abasic (AP) Site Dynamics To Facilitate AP-Lyase Reactions. [Abstract]2023 May 19;18(5):1168-1179. PMID: 36930463 -
Dig Dis Sci
Dihydroartemisinin: A Targeting Agent of CCT3-Mediated Oxidative Stress to Enhance the Antitumor Activity of Doxorubicin in Hepatocellular Carcinoma. [Abstract]2025 Nov 8. PMID: 41205108 -
Toxicon
A Dendrobium chrysotoxum extract erianin induce AML cells death by activating PPARɑ and downregulating PI3K/AKT signaling pathways. [Abstract]2025 Jul:261:108371. PMID: 40280444 -
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Solvent & Solubility
H2O : 10 mg/mL (Need ultrasonic)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)