Fluorescence in situ hybridization detects specific DNA or RNA sequences inside fixed cells or tissue sections by hybridizing fluorescently labeled nucleic-acid probes to complementary target sequences, allowing the target’s copy number, chromosomal position, spatial distribution, or transcript abundance to be visualized microscopically. DNA-FISH detects genomic loci, chromosomal gains/losses, amplifications, deletions, and rearrangements, while RNA-FISH detects RNA molecules or transcript localization; in cancer cells, mouse tumors, neurons, organoids, macrophages, or drug-screening samples, the readout is fluorescent puncta, fusion/split signals, or localized RNA signal interpreted relative to validated controls.
Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a molecular biology technique used to locate specific nucleic acid sequences in cells or tissues. By using fluorescently labeled nucleic acid probes, FISH allows researchers to directly visualize and localize DNA or RNA. This technology is widely used in chromosome analysis, gene mapping, karyotype analysis and other fields. FISH not only provides high spatial resolution but also allows for simultaneous detection of multiple targets, making it a powerful tool in biomedical research, clinical diagnostics, and cancer research.
Related Experimental Schemes
