Sperabillins, new antibacterial antibiotics with potent in vivo activity. Taxonomy, fermentation, isolation and biological activity

  • J Antibiot (Tokyo). 1992 Jan;45(1):10-9. doi: 10.7164/antibiotics.45.10.
N Katayama  1 ,  Y Nozaki ,  S Tsubotani ,  M Kondo ,  S Harada ,  H Ono
Affiliations
  • 1. Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
PMID: 1372306 DOI: 10.7164/antibiotics.45.10
Abstract

A Gram-negative bacterium was found to produce new Antibacterial antibiotics, sperabillins A, B, C and D, and the producing bacterium was characterized and identified as Pseudomonas fluorescens YK-437. Sperabillins were isolated by column chromatographies using cation-exchange resins, activated carbon and cation-exchange Sephadex, and preparative reverse-phase HPLC. Sperabillins showed Antibacterial activity against Gram-negative and Gram-positive bacteria including antibiotic-resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. Sperabillin A inhibited DNA, RNA, protein, and cell wall biosynthesis in Escherichia coli. Sperabillins showed good protective effects in experimentally infected mice.

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