Synthesis of a caged glutamate for efficient one- and two-photon photorelease on living cells

  • Chem Commun (Camb). 2005 Aug 7;(29):3664-6. doi: 10.1039/b504922a.
Olesya D Fedoryak  1 ,  Jai-Yoon Sul ,  Philip G Haydon ,  Graham C R Ellis-Davies
Affiliations
  • 1. Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
PMID: 16027904 DOI: 10.1039/b504922a
Abstract

We have synthesized a biologically inert, photosensitive derivative of the major excitatory amino acid, L-glutamate (which we call MDNI-glu) that makes more efficient use of incident light than all Other caged glutamates. Laser flash photolysis of MDNI-glu in acutely isolated hippocampal brain slices evoked a rapid increase in intracellular Ca2+ concentration in astrocytes.

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