Proteomic analysis to dissect mitoxantrone resistance-associated proteins in a squamous lung carcinoma
- Anticancer Res. 2007 May-Jun;27(3A):1277-84.
- 1. National Institute of Cellular Biology, Dublin City University, Ireland.
Background: Mitoxantrone resistance has been related to the expression of a drug efflux pump Breast Cancer resistance pump (BCRP) but little is known of the intracellular protein changes. In this work, differential protein expression in a squamous lung carcinoma cell line, DLKP, and its mitoxantrone-resistant variant (DLKP-Mitox) was investigated to elucidate other changes associated with mitoxantrone resistance.
Materials and methods: Differential protein expression between DLKP and DLKP-Mitox was investigated using 2D-DIGE technology. Proteins of interest were identified by MALDI-ToF mass spectrometry. Western blotting was used to confirm and validate some of these changes.
Results: Biological variation analysis in Decyder software revealed a total of 343 proteins to be differentially regulated with p < 0.05. Identification of 61 proteins of interest by mass spectrometry revealed changes in proteins involved in many cellular processes including Apoptosis and differentiation.
Conclusion: Alterations in these cellular processes and proteins present alternative sites to circumvent resistance to mitoxantrone.