Jingzhaotoxin-XII, a gating modifier specific for Kv4.1 channels

  • Toxicon. 2007 Oct;50(5):646-52. doi: 10.1016/j.toxicon.2007.05.009.
Chunhua Yuan  1 ,  Zhi Liao ,  Xiongzhi Zeng ,  Longjun Dai ,  Fang Kuang ,  Songping Liang
Affiliations
  • 1. Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, Life Science College, Hunan Normal University, Changsha 410081, PR China.
PMID: 17631373 DOI: 10.1016/j.toxicon.2007.05.009
Abstract

Jingzhaotoxin-XII (JZTX-XII), a 29-residue polypeptide, was purified from the venom of the Chinese tarantula Chilobrachys jingzhao. Electrophysiological recordings carried out in Xenopus laevis oocytes showed that JZTX-XII is specific for Kv4.1 channels, with the IC50 value of 0.363 microM. It interacts with the channels by modifying the gating behavior. JZTX-XII shares 80% sequence identity with phrixotoxin1, a potent inhibitor for Kv4.2 and Kv4.3 channels. Structure analysis indicates that the difference of the charge distribution in the interactive surface perhaps influences the specific pharmacology of the toxins. JZTX-XII should be a valuable tool for the investigation of the Kv4.1 channels.

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