Efficient genome editing in zebrafish using a CRISPR-Cas system

  • Nat Biotechnol. 2013 Mar;31(3):227-9. doi: 10.1038/nbt.2501.
Woong Y Hwang  1 ,  Yanfang Fu ,  Deepak Reyon ,  Morgan L Maeder ,  Shengdar Q Tsai ,  Jeffry D Sander ,  Randall T Peterson ,  J-R Joanna Yeh ,  J Keith Joung
Affiliations
  • 1. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
PMID: 23360964 DOI: 10.1038/nbt.2501
Abstract

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 Endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.