Purification and culture of dorsal root ganglion neurons

  • Cold Spring Harb Protoc. 2014 Aug 1;2014(8):813-4. doi: 10.1101/pdb.top073965.
J Bradley Zuchero  1
Affiliations
  • 1. Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305.
PMID: 25086024 DOI: 10.1101/pdb.top073965
Abstract

Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.