Bio-nanogate controlled enzymatic reaction for virus sensing

  • Biosens Bioelectron. 2015 May 15:67:400-7. doi: 10.1016/j.bios.2014.08.071.
Ronghui Wang  1 ,  Lizhou Xu  2 ,  Yanbin Li  3
Affiliations
  • 1. Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
  • 2. Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China.
  • 3. Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: [email protected].
PMID: 25212377 DOI: 10.1016/j.bios.2014.08.071
Abstract

The objective of this study was to develop an aptamer-based bifunctional bio-nanogate, which could selectively respond to target molecules, and control enzymatic reaction for electrochemical measurements. It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of Other viruses.

Keywords
Aptamer; Avian influenza; Bio-nanogate; Enzymatic reaction; Virus sensing.
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