Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice

  • J Nutr Biochem. 2015 Jan;26(1):57-63. doi: 10.1016/j.jnutbio.2014.09.004.
Kazuaki Yoshinaga  1 ,  Keiichi Sasaki  2 ,  Hiroyuki Watanabe  3 ,  Koji Nagao  4 ,  Nao Inoue  4 ,  Bungo Shirouchi  4 ,  Teruyoshi Yanagita  5 ,  Toshiharu Nagai  6 ,  Hoyo Mizobe  6 ,  Koichi Kojima  6 ,  Fumiaki Beppu  2 ,  Naohiro Gotoh  7
Affiliations
  • 1. Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan; Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
  • 2. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
  • 3. Department of Health Science, University of Kochi, 2751-1 Ike, Kochi-shi, Kochi 781-0111, Japan.
  • 4. Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga-shi, Saga 840-8502, Japan.
  • 5. Department of Health and Nutrition Science, Nishikyushu University, 4490-9 Ozaki, Kanzaki-machi, Kanzaki-shi, Saga 842-8585, Japan.
  • 6. Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
  • 7. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: [email protected].
PMID: 25448607 DOI: 10.1016/j.jnutbio.2014.09.004
Abstract

The present study investigated the effects of binding position of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to triacylglycerol (TAG) on lipid metabolism in C57BL/6J mice. Mice were treated with pure TAG positional isomers, including 1,2(2,3)-dipalmitoyl-3(1)-eicosapentaenoyl glycerol, 1,3-dipalmitoyl-2-eicosapentaenoyl glycerol, 1,2(2,3)-dipalmitoyl-3(1)-docosahexaenoyl glycerol, and 1,3-dipalmitoyl-2-docosahexaenoyl glycerol. Compared to DHA bound to the α-position of TAG, DHA bound to the β-position more effectively inhibited fatty acid synthetic Enzymes and cholesterol-metabolism Enzymes and thus reduced TAG and Cholesterol concentrations in the serum and liver. EPA bound to the α-position of TAG, but not EPA bound to the β-position of TAG, significantly decreased hepatic Cholesterol concentrations. Additionally, EPA bound to the α-position of TAG increased the ratio of PGI2 to TXA2 to a higher degree than EPA bound to the β-position. These results suggested that the binding position of EPA and DHA to TAG affected TAG and Cholesterol metabolism as well as eicosanoid production in C57BL/6J mice.

Keywords
C57BL/6J mouse; Docosahexaenoic acid; Eicosapentaenoic acid; Lipid metabolism; Positional isomer; Triacylglycerol.
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