Hypocrellin B and paclitaxel-encapsulated hyaluronic acid-ceramide nanoparticles for targeted photodynamic therapy in lung cancer

  • J Photochem Photobiol B. 2016 May:158:113-21. doi: 10.1016/j.jphotobiol.2016.02.035.
Ji-Eun Chang  1 ,  Hyun-Jong Cho  2 ,  Eunjue Yi  1 ,  Dae-Duk Kim  3 ,  Sanghoon Jheon  4
Affiliations
  • 1. Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam-Si, Gyeonggi-do, Republic of Korea.
  • 2. College of Pharmacy, Kangwon National University, Chuncheon, Republic of Korea.
  • 3. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea.
  • 4. Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Seongnam-Si, Gyeonggi-do, Republic of Korea; Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: [email protected].
PMID: 26967521 DOI: 10.1016/j.jphotobiol.2016.02.035
Abstract

To increase the therapeutic efficacy of photodynamic therapy (PDT) in treating lung Cancer, we developed both Photosensitizer and Anticancer drug encapsulated hyaluronic acid-ceramide nanoparticles. Based on our previous study, a co-delivery system of photosensitizers and Anticancer agents greatly improves the therapeutic effect of PDT. Furthermore, hyaluronic acid-ceramide-based nanoparticles are ideal targeting carriers for lung Cancer. In vitro phototoxicity in A549 (human lung adenocarcinoma) cells and in vivo antitumor efficacy in A549 tumor-bearing mice treated with hypocrellin B (HB)-loaded nanoparticles (HB-NPs) or hypocrellin B and paclitaxel loaded nanoparticles (HB-P-NPs) were evaluated. Cell viability assay, microscopic analysis and FACS analysis were performed for the in vitro studies and HB-P-NPs showed enhanced phototoxicity compared with HB-NPs. In the animal study, the tumor volume change and the histological analysis was studied and the Anticancer efficacy improved in the order of free HB<HB-NPs<HB-P-NPs. In conclusion, the combination therapy of PDT and chemotherapy, and hyaluronic acid-ceramide nanoparticle-based targeted delivery improved the effects of PDT in lung Cancer in mice.

Keywords
Hyaluronic acid–ceramide nanoparticle; Hypocrellin B; Lung cancer; Paclitaxel; Photodynamic therapy.
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