Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time

  • FEBS Lett. 2016 Sep;590(18):3051-60. doi: 10.1002/1873-3468.12365.
Christie A Bader  1 ,  Alexandra Sorvina  1 ,  Peter V Simpson  2 ,  Phillip J Wright  2 ,  Stefano Stagni  3 ,  Sally E Plush  1 ,  Massimiliano Massi  2 ,  Douglas A Brooks  4
Affiliations
  • 1. Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia.
  • 2. Department of Chemistry and Nanochemistry Research Institute, Curtin University, Bently, Australia.
  • 3. Department of Industrial Chemistry 'Toso Montanari', University of Bologna, Italy.
  • 4. Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia. [email protected].
PMID: 27543777 DOI: 10.1002/1873-3468.12365
Abstract

Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3 (1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER(™) , shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER(™) localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.

Keywords
endoplasmic reticulum; live cell imaging; rhenium.
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