Periodic Acid-Schiff Staining with Diastase

  • Methods Mol Biol. 2017:1639:145-149. doi: 10.1007/978-1-4939-7163-3_14.
Dongtao A Fu  1 ,  Martha Campbell-Thompson  2
Affiliations
  • 1. Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA.
  • 2. Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA. [email protected].
PMID: 28752454 DOI: 10.1007/978-1-4939-7163-3_14
Abstract

Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.

Keywords
Alpha-1 antitrypsin deficiency; Human liver; Periodic Acid-Schiff; Periodic Acid-Schiff reaction-diastase; Special stain.