Structural Characterization of Terpenoids from Abies holophylla Using Computational and Statistical Methods and Their Biological Activities

  • J Nat Prod. 2018 Aug 24;81(8):1795-1802. doi: 10.1021/acs.jnatprod.8b00245.
Chung Sub Kim  1 ,  Joonseok Oh  2  3 ,  Lalita Subedi  4  5 ,  Sun Yeou Kim  4  5 ,  Sang Un Choi  6 ,  Kang Ro Lee  1
Affiliations
  • 1. Natural Products Laboratory, School of Pharmacy , Sungkyunkwan University , Suwon 16419 , Republic of Korea.
  • 2. Department of Chemistry , Yale University , New Haven , Connecticut 06520 , United States.
  • 3. Chemical Biology Institute , Yale University , West Haven , Connecticut 06516 , United States.
  • 4. Gachon Institute of Pharmaceutical Science , Gachon University , Incheon 21936 , Republic of Korea.
  • 5. College of Pharmacy , Gachon University , #191, Hambakmoero , Yeonsu-gu, Incheon 21936 , Republic of Korea.
  • 6. Korea Research Institute of Chemical Technology , Daejeon 34114 , Republic of Korea.
PMID: 30063340 DOI: 10.1021/acs.jnatprod.8b00245
Abstract

Three new Diterpenoids (1-3) and three new triterpenoids (4-6) were isolated from the trunk of Abies holophylla together with 19 known Terpenoids. The chemical structures of 1-6 were determined through NMR and MS data analyses. Also, the structural assignments of some of these compounds were verified and elucidated utilizing computational methods coupled with a statistical procedure (CP3, DP4, and DP4+). All the isolated compounds were evaluated for their cytotoxicity against four human Cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15). In addition, the compounds were tested for their anti-inflammatory effects in lipopolysaccharide-stimulated murine microglia BV2 cells by measuring nitric oxide levels, and for their neuroprotective activity in C6 cells through induction of nerve growth factor.