miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR

  • Onco Targets Ther. 2018 Aug 30;11:5293-5302. doi: 10.2147/OTT.S173416.
Lansheng Zhang  1  2 ,  Yanwei Chen  3 ,  Hui Wang  3 ,  Xia Zheng  2 ,  Caihong Li  2 ,  Zhengxiang Han  3
Affiliations
  • 1. Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
  • 2. Department of Radiation Oncology, the Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, People's Republic of China.
  • 3. Department of Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, People's Republic of China, [email protected].
PMID: 30214235 DOI: 10.2147/OTT.S173416
Abstract

Background: The roles and related mechanism of miR-376a in Breast Cancer cell progression are unclear.

Methods: Kaplan-Meier plotter analysis was used to analyze the correlation between miR-376a and the overall survival (OS) of Breast Cancer patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect miR-376a level in Breast Cancer cells. Cell viability, transwell migration and invasion, and cell Apoptosis were constructed to investigate the effects of miR-376a on Breast Cancer cells. Luciferase reporter and RNA immunoprecipitation (RIP) were used to explore the targeting of miR-376a on NRP-1.

Results: miR-376a expression was positively correlated with the overall survival of Breast Cancer patients, and significantly decreased in Breast Cancer cells. Functionally, miR-376a over-expression suppressed cell proliferation, migration and invasion, and promoted cells Apoptosis. Additionally, miR-376a could directly target NRP-1 and exerted its effect through NRP-1.

Conclusion: miR-376a could suppress Breast Cancer cell progression via directly targeting NRP-1.

Keywords
NRP-1t; Wn/β-catenin migration; breast cancert; miR-376at.
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