One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets

  • ACS Omega. 2019 Aug 12;4(9):13790-13794. doi: 10.1021/acsomega.9b01321.
Rohit Varshney  1 ,  Shubhra Sharma  2 ,  Bhanu Prakash  1 ,  Joydev K Laha  2 ,  Debabrata Patra  1
Affiliations
  • 1. Habitat Centre, Institute of Nano Science and Technology, Phase-10, Sector-64, Mohali, Punjab 160062, India.
  • 2. Department of Pharmaceutical Technology (Process Chemistry), National Institute of Pharmaceutical Education and Research, S. A. S. Nagar, Mohali, Punjab 160062, India.
PMID: 31497697 DOI: 10.1021/acsomega.9b01321
Abstract

Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling Enzymes at the oil-water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle-catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a K m value of 42 mM and shows 1.1-fold higher activity compared to free Enzymes. Finally, the robust MCs showed excellent recyclability, which can meet the demand of industrial biotechnological applications.