Inverse-micelle synthesis of doxorubicin-loaded alginate/chitosan nanoparticles and in vitro assessment of breast cancer cytotoxicity

  • Colloid Interface Sci Commun. 2019 Jan;28:69-74. doi: 10.1016/j.colcom.2018.12.002.
Justin G Rosch  1 ,  Hayden Winter  2 ,  Allison N DuRoss  1 ,  Gaurav Sahay  1  3 ,  Conroy Sun  1  4
Affiliations
  • 1. Department of Pharmaceutical Science, Oregon State University, 2730 SW Moody Ave, Portland, OR, 97201, USA.
  • 2. Department of Chemistry, Portland State University, 1719 SW 10th Ave, Portland, OR, 97201, USA.
  • 3. Department of Biomedical Engineering, 2730 SW Moody Ave, Oregon Health & Science University, Portland, OR 97201, USA.
  • 4. Department of Radiation Medicine, 3181 S.W. Sam Jackson Park Rd, Oregon Health & Science, University, Portland, OR 97239, USA.
PMID: 31602357 DOI: 10.1016/j.colcom.2018.12.002
Abstract

Naturally-derived Polysaccharides, such as alginate and chitosan, can be assembled to form nanocarriers for the delivery of therapeutic agents. Here we exploit the electrostatic complexation of alginate/chitosan in a water-in-oil (w/o) emulsion process to produce doxorubicin (DOX)-loaded nanoparticles (~80 nm) with exceptional spherical morphology and uniformity. This robust synthetic route utilizes an aqueous phase dispersed in a cyclohexane/dodecylamine organic phase and is capable of encapsulating DOX in the nanoparticle solution. The uptake and efficacy of this novel formulation was evaluated in a murine breast Cancer cell line, 4T1, with comparable 72 h IC50 values of the nanoparticle solution (0.15 μg/mL) and free DOX (0.13 μg/mL). Overall, the favorable performance, physiochemical properties, and their facile production support these nanocarriers as promising platform for the delivery of aqueous soluble drugs.

Keywords
alginate; breast cancer; cell viability; chitosan; doxorubicin.
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