Potential Myocardial Protection of 3,4-seco-Lupane Triterpenoids from Acanthopanax sessiliflorus Leaves

  • Chem Biodivers. 2021 Jan;18(1):e2000830. doi: 10.1002/cbdv.202000830.
Haohao Wang  1 ,  Chen Chen  1 ,  Ruijuan Liu  2 ,  Xu Wang  1 ,  Yan Zhao  1 ,  Zhaowei Yan  2 ,  Enbo Cai  1 ,  Hongyan Zhu  1
Affiliations
  • 1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, P. R. China.
  • 2. Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P. R. China.
PMID: 33188566 DOI: 10.1002/cbdv.202000830
Abstract

A rich of 3,4-seco-lupane triterpenoids including chiisanoside (CSS), divaroside (DVS), sessiloside-A1 (SSA) and chiisanogenin (CSG) were isolated from the ethanol extract of the leaves of Acanthopanax sessiliflorus. On the basis of previous studies, this article focused on four important components of 3,4-seco-lupane triterpenoids in Acanthopanax sessiliflorus leaves and explored their protective effects against aconitine-induced cardiomyocyte injury and their molecular mechanisms. The results showed that pretreatment with 3,4-seco-lupane triterpenoids could effectively increase cell viability, reduce CK-MB and LDH activities, reduce ROS production, maintain calcium concentration balance, and inhibit Apoptosis, with divaroside having the best effect. In addition, Western blot results showed that divaroside down-regulated Cleaved Caspase-3 and Bax and up-regulated Bcl-2 expression through activating the PI3 K/Akt pathway. However, the LY294002 inhibitor reversed this situation. This suggests that 3,4-seco-lupane triterpenoids may be a new hotspot for potential myocardial protective drugs research.

Keywords
3,4-seco-lupane triterpenoids; Acanthopanax sessiliflorus; PI3 K/AKT signal pathway; myocardial injury.
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