MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway

  • J Cancer. 2021 Jan 1;12(2):467-473. doi: 10.7150/jca.49641.
Tonglei Chen  1 ,  Zhenshi Chen  2  3 ,  Xiaoning Lian  4 ,  Weidong Wu  2  3 ,  Lei Chu  2  3 ,  Shaoru Zhang  2  3 ,  Lihui Wang  2  3
Affiliations
  • 1. Suzhou Ninth People's Hospital, Suzhou, Jiangsu 215200, China.
  • 2. Danyang People's Hospital of Jiangsu Province, Danyang, Jiangsu 212300, China.
  • 3. Danyang Hospital Affiliated to Nantong University, Danyang, Jiangsu 212300, China.
  • 4. Department of Assay Development, TOT BIOPHARM co., LTD, Suzhou, Jiangsu 215024, China.
PMID: 33391443 DOI: 10.7150/jca.49641
Abstract

Objective: To investigate the high expression of MUC15 in promoting proliferation, migration and invasion in Osteosarcoma (OS) cell and its potential mechanism. Methods: The expressions of MUC15 in OS patients were analyzed from GEO Datasets, tumor cell lines and clinical samples. The roles of MUC15 in OS were explored by CCK-8, flow cytometry, transwell and western blot assay, respectively. Results: MUC15 was highly expressed in Osteosarcoma, and there was a significant negative correlation between MUC15 and the prognosis. Knockdown of MUC15 in HOS and U-2OS could promote tumor cell Apoptosis, down-regulate the expression of MMP2/9, reduce the epithelial interstitial transition and silence the Wnt/b-Catenin signal pathway. Conclusion: The high-expression of MUC15 promotes the proliferation, migration and invasion of Osteosarcoma through anti-apoptosis, increasing the invasive ability by epithelial interstitial transition, and activating the Wnt/b-Catenin signal pathway.

Keywords
MUC 15; Osteosarcoma; invasion; migration; proliferation.
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