A Novel Procedure for Orthotopic Tibia Implantation for Establishment of a More Clinical Osteosarcoma PDOX Mouse Model
- In Vivo. 2021 Jan-Feb;35(1):105-109. doi: 10.21873/invivo.12237.
- 1. AntiCancer Inc, San Diego, CA, U.S.A.
- 2. Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, U.S.A.
- 3. Department of Surgery, University of California, San Diego, CA, U.S.A.
- 4. AntiCancer Inc, San Diego, CA, U.S.A.; [email protected].
Background/aim: Osteosarcoma is a rare type of malignancy that affects mostly children and adolescents. A new procedure was designed to create an improved patient-derived orthotopic xenograft (PDOX) mouse model of Osteosarcoma that more closely mimics Osteosarcoma in clinical settings. Previous Osteosarcoma PDOX models involved implanting a tumor fragment near the femur of nude mice in a space created by separating muscle.
Materials and methods: A hole was created in the tibia of nude mice and an Osteosarcoma tumor fragment was implanted directly into the bone.
Results: This procedure resulted in tumor growth in the bone similar to Osteosarcoma tumors found in clinical patients.
Conclusion: The establishment ratio for this procedure is 80% making it a practical and clinically-relevant model for screening effective therapies for Osteosarcoma patients.