Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

  • STAR Protoc. 2021 Feb 8;2(1):100333. doi: 10.1016/j.xpro.2021.100333.
Caroline Perner  1 ,  Caroline L Sokol  1
Affiliations
  • 1. Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
PMID: 33615276 DOI: 10.1016/j.xpro.2021.100333
Abstract

In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as a possible downstream application. In addition, isolated DRGs can be used directly for immunofluorescence, flow cytometry, RNA Sequencing or proteomic approaches, electrophysiology, and calcium imaging. For complete details on the use and execution of this protocol, please refer to Perner et al. (2020).

Keywords
Cell culture; Cell isolation; Cell-based assays; Neuroscience.