Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis

  • Oxid Med Cell Longev. 2022 Aug 24:2022:5142381. doi: 10.1155/2022/5142381.
Lili Shi  1 ,  Yuliang Li  2 ,  Meixin Shi  3 ,  Xiaoxue Li  3 ,  Guopeng Li  3 ,  Jie Cen  3 ,  Dan Liu  4 ,  Can Wei  3 ,  Yan Lin  5
Affiliations
  • 1. Department of Cadre Ward, The First Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
  • 2. Department of Anesthesiology, The Fifth Hospital of Harbin, Harbin 150081, China.
  • 3. Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
  • 4. Department of Cadre Ward, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
  • 5. Department of Pathophysiology, Qiqihar Medical University, Qiqihar 161006, China.
PMID: 36062192 DOI: 10.1155/2022/5142381
Abstract

Background: It is well-known that dysfunctions of vascular smooth muscle cells (VSMCs) act an essential part in vascular complications of diabetes. Studies have shown that circular RNAs (circRNAs) and MicroRNAs (miRNAs) play a crucial role in regulating cell functions. However, their influence on the proliferation, calcification, and Autophagy of VSMCs remains to be further explored. Therefore, this study elucidates the role and mechanism of hsa_circRNA_0008028 in high glucose- (HG-, 30 mM) treated VSMCs in vitro.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was chosen to detect the levels of hsa_circRNA_0008028, miR-182-5p, and tribble 3 (TRIB3). Then, dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to predict and verify the binding relationship between miR-182-5p and hsa_circRNA_0008028 or TRIB3. Cell counting kit-8 assay, 5-ethynyl-2'-deoxyuridine (EdU) staining, corresponding commercial kits, and western blotting were used to measure indexes reflecting cell viability, proliferation, calcification, and Autophagy of VSMCs, respectively.

Results: In HG-induced VSMCs, hsa_circRNA_0008028 and TRIB3 were highly expressed, whereas miR-182-5p decreased. Meanwhile, cell proliferation, calcification, and Autophagy could be repressed by silencing of hsa_circRNA_0008028. However, these effects can be eliminated by miR-182-5p inhibition. Furthermore, it was demonstrated that hsa_circRNA_0008028 could promote the expression of TRIB3, a target of miR-182-5p, by directly sponging miR-182-5p. The expression of TRIB3 was suppressed by hsa_circRNA_0008028 knockout, which was rescued by miR-182-5p inhibition.

Conclusion: This study reveals that hsa_circRNA_0008028 can act as a Sponge of miR-182-5p and promote HG-induced proliferation, calcification, and Autophagy of VSMCs partly by regulating TRIB3.

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