Analytical study of kallikrein and kallikrein-like esterase activity in subfractions from rat kidney cortex microsomes and isolated subcellular membranes

  • Adv Exp Med Biol. 1986;198 Pt A:355-9. doi: 10.1007/978-1-4684-5143-6_48.
K J Andersen ,  J Ofstad
PMID: 3643716 DOI: 10.1007/978-1-4684-5143-6_48
Abstract

Heavy and light microsomal fractions were subfractioned using high performance zonal rotors, and assayed for apoprotenin sensitive kallikrein-like amidolytic activity (pH 8.2). The activity profiles for the various substrates assayed show rather complex distribution pattern demonstrating kallikrein-like amidolytic activity in plasma membranes, basolateral membranes, rough endoplasmic reticulum and membranes derived from the Golgi complex.

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