Genistein Induces Antiproliferative Activity and Apoptosis in Human Osteosarcoma Saos-2 Cells
- Anticancer Res. 2023 Dec;43(12):5387-5392. doi: 10.21873/anticanres.16742.
- 1. Department of Biomedical Engineering, Toin University of Yokohama, Yokohama, Japan [email protected].
- 2. Department of Biomedical Engineering, Toin University of Yokohama, Yokohama, Japan.
Background/aim: Genistein (4', 5, 7-trihydroxyisoflavone) and daidzein (4', 7-dihydroxyisoflavone) are Isoflavones derived from soybean and have anti-cancer effects in various cells. However, the effects of genistein and daidzein on the human Osteosarcoma cell line Saos-2 has not been investigated before.
Materials and methods: Human Osteosarcoma Saos-2 cells were treated with genistein for 24 and 48 hours. Cytotoxicity and Apoptosis were measured.
Results: Genistein significantly inhibited proliferation of Saos-2 cells stronger than daidzein in a dose-dependent manner (0 to 80 μM). Genistein also significantly suppressed Saos-2 cell viability in a dose-dependent manner (0 to 100 μM). In contrast, daidzein did not affect Saos-2 cell viability. Real-Time PCR revealed that genistein caused G1-arrest by increasing the expression of p21 and p27 mRNAs in Saos-2 cells. In addition, genistein induced Apoptosis through the up-regulation of effector Caspase-3/7 activity in Saos-2 cells. Genistein also enhanced initiator caspase-9 and TNF-α mRNA expression in cells.
Conclusion: Genistein may inhibit proliferation through the up-regulation of p21 and p27 and viability by inducing Apoptosis in Saos-2 cells.