Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication

  • J Virol. 2024 Jan 5:e0184223. doi: 10.1128/jvi.01842-23.
Shuang-Shuang Zhao  1  2 ,  Qisheng Qian  1  2 ,  Xin-Xin Chen  2 ,  Qingxia Lu  2 ,  Guangxu Xing  2 ,  Songlin Qiao  2 ,  Rui Li  2 ,  Gaiping Zhang  1  2  3
Affiliations
  • 1. College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.
  • 2. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
  • 3. Longhu Modern Immunology Laboratory, Zhengzhou, Henan, China.
PMID: 38179942 DOI: 10.1128/jvi.01842-23
Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) Infection results in a serious swine disease affecting pig farming worldwide. Despite that numerous studies have shown that PRRSV triggers Autophagy for its self-replication, how PRRSV induces Autophagy is incompletely understood. Here, we identify that PRRSV Nsp2 degrades GRASP65 to induce GA fragmentation, which dissociates RAB2 from GM130 and activates RAB2-ULK1-mediated Autophagy to enhance viral replication. This work expands our understanding of PRRSV-induced Autophagy and PRRSV replication, which is beneficial for anti-viral drug development.

Keywords
GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1; autophagy.
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