Huaier Regulates Oxaliplatin Resistance in Colorectal Cancer by Regulating Autophagy and Inhibiting the Wnt/β-catenin Signalling Pathway

  • Front Biosci (Landmark Ed). 2024 Jan 17;29(1):15. doi: 10.31083/j.fbl2901015.
Qi Feng  1 ,  Mingyi Huo  1 ,  Xiaoli Yan  1 ,  Luwen Wen  1 ,  Jining Zheng  1
Affiliations
  • 1. Department of Pathophysiology, Chengde Medical University, 067000 Chengde, Hebei, China.
PMID: 38287798 DOI: 10.31083/j.fbl2901015
Abstract

Objective: The present study aims to investigate the effect of Huaier on oxaliplatin (OXA) resistance in HCT-8 colorectal Cancer (CRC) cells.

Methods: Oxaliplatin-resistant HCT-8/L CRC cells were used. The Cell Counting Kit-8, western blotting, quantitative real-time polymerase chain reaction, protein extraction kit, immunofluorescence and acridine orange staining assays were used in the study. The experiment results proved that Huaier has an influence on the Wnt/β-catenin signalling pathway, Autophagy and drug resistance. The authors of the present study used chloroquine, an Autophagy inhibitor and Wnt agonist 1 (a Wnt pathway agonist) to verify the present experiment.

Results: The results showed that Huaier can regulate Autophagy, inhibit the Wnt/β-catenin signalling pathway and reverse the drug resistance of OXA-resistant CRC cells.

Conclusions: This study proved that Huaier can regulate Autophagy, inhibit the Wnt/β-catenin signalling pathway and reverse the drug resistance of OXA-resistant CRC cells.

Keywords
Huaier; Wnt/β-catenin; autophagy; colorectal cancer; drug resistance.
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