Mir-615-5p inhibits cervical cancer progression by targeting TMIGD2

  • Hereditas. 2025 Jan 9;162(1):4. doi: 10.1186/s41065-024-00363-7.
Yan Zhao  1 ,  Haitao Chen  2 ,  Wenhui Zhang  3 ,  Wei Shang  4 ,  Jinwei Cao  5 ,  Huijuan Zhao  6 ,  Zhixiang Zou  7
Affiliations
  • 1. Department of Gynecology, Taiyuan Central Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China.
  • 2. Department of Obstetrics and Gynecology, Zhucheng Hospital of Traditional Chinese Medicine, Weifang, 262200, Shandong, China.
  • 3. Department of Medical Imaging, The Third Hospital of Shijiazhuang, No. 15, Tiyu South Street, Chang'an District, Shijiazhuang, 050051, Hebei, China. [email protected].
  • 4. Department of Obstetrics and Gynecology, Renqiu People's Hospital, Renqiu, 062550, Hebei, China.
  • 5. Department of Medical Imaging, The Sixth Hospital of Shijiazhuang, Shijiazhuang, 050051, Hebei, China.
  • 6. Second Department of Obstetrics and Gynecology, The Sixth Hospital of Shijiazhuang, Shijiazhuang, 050051, Hebei, China.
  • 7. Obstetrics and Gynecology Medical Centre, The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, No.105, Shaoshan Middle Road, Yuhua District, Changsha, 410007, Hunan, China. [email protected].
PMID: 39789663 DOI: 10.1186/s41065-024-00363-7
Abstract

Background: Cervical Cancer (CC) is a prevalent gynecological malignancy, contributing to a substantial number of fatalities among women. MicroRNAs (miRNAs) have emerged as promising biomarkers with significant potential for the early detection and prognosis of CC.

Objective: This study aimed to explore the clinical significance and biological role of miR-615-5p in CC, with the goal of identifying novel biomarkers for this disease.

Materials and methods: The levels of miR-615-5p and TMIGD2 mRNA in tissue samples and cells were quantified through quantitative reverse transcription Real-Time PCR, followed by statistical analyses to investigate the correlation between miR-615-5p and clinical data. The effects of miR-615-5p on the proliferation and metastasis of CC cells were evaluated using the Cell Counting Kit-8 and Transwell assays. The potential mechanism of miR-615-5p was elucidated by bioinformatics analyses and Dual-luciferase reporter assay. Western blotting was employed to measure the protein levels of TMIGD2.

Results: In CC, the downregulation of miR-615-5p was related to poor prognosis and emerged as an independent prognostic factor. The levels of miR-615-5p were reduced in CC cells. miR-615-5p overexpression restrained the proliferation and metastasis of CC cells. Furthermore, TMIGD2 was identified as a target gene regulated by miR-615-5p, and its expression was notably elevated in CC. The influence of miR-615-5p on the biological behaviors of CC cells was mediated through the modulation of TMIGD2.

Conclusions: Downregulation of miR-615-5p was a prognostic indicator of poor prognosis in CC. miR-615-5p exerted its tumor-suppressive effects by inhibiting cell growth and metastasis through the regulation of TMIGD2.

Keywords
Cervical cancer; Prognosis; Progression; TMIGD2; miR-615-5p.
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