A novel glucomannan from Bletilla striata ameliorates colitis: Restores intestinal barrier, alleviates inflammation, and modulates the gut flora

  • Int J Biol Macromol. 2025 Sep;321(Pt 3):146421. doi: 10.1016/j.ijbiomac.2025.146421.
Xialin Sun  1 ,  Tingwen Zhang  1 ,  Yan Zhao  2 ,  Han Yang  1 ,  Yawei Li  3 ,  Xin Sun  4 ,  Xingbo Bian  5
Affiliations
  • 1. School of Pharmaceutical Sciences, Jilin Medical University, China.
  • 2. College of Chinese Medicinal Materials, Jilin Agricultural University, China.
  • 3. School of Pharmaceutical Sciences, Jilin Medical University, China. Electronic address: [email protected].
  • 4. School of Pharmaceutical Sciences, Jilin Medical University, China. Electronic address: [email protected].
  • 5. School of Pharmaceutical Sciences, Jilin Medical University, China. Electronic address: [email protected].
PMID: 40752704 DOI: 10.1016/j.ijbiomac.2025.146421
Abstract

Background: Ulcerative colitis (UC) involves intestinal barrier dysfunction, immune dysregulation, and microbiota imbalance. Bletilla striata polysaccharide (BSP) shows anti-inflammatory potential, but its UC mechanisms remain unclear.

Purpose: To purify Bletilla striata polysaccharide (BSP-1), characterize its structure, and elucidate its multi-target UC therapeutic mechanisms.

Methods: This study purified a novel structure of neutral glucomannan with a small amount of acetylation (BSP-1). Together with tight junction protein analysis, oxidative stress markers, 16S rRNA Sequencing, cytokine quantification, and molecular ecological network examination, the team used a UC mouse model created using dextran sodium sulfate (DSS).

Results: BSP-1 alleviated colitis by inhibiting TLR4/MyD88/NF-κB signaling (elevating IL-10; lowering IL-6, TNF-α, and IL-1β) and restoring integrity of the intestinal barrier via upregulating Occludin, ZO-1, MUC2, and Claudin-1. It reduced oxidative stress (lower MDA; higher SOD) and modulated gut microbiota (enriched Lactobacillus, suppressed Escherichia-Shigella). Molecular ecological network analysis showed that BSP-1 restored microbial community stability by enhancing Bacterial competition.

Conclusion: BSP-1 exerts integrated anti-inflammatory, barrier-repairing, and microbiota-regulating effects, highlighting its potential as a multi-target UC therapy and intestinal microecological regulator.

Keywords
Bletilla striata polysaccharide; Gut microbiota; Ulcerative colitis.
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