The Kininogen 1/bradykinin receptor B2 system serves as a key molecular response of vancomycin-induced nephrotoxicity and may be a potential target of absinthin
- Histol Histopathol. 2026 May 5:25085. doi: 10.14670/HH-25-085.
- 1. Physical Examination Center, Guangzhou Eighth People's Hospital, Guanzhou Medical University, Baiyun District, Guangzhou City, Guangdong Province, China.
- 2. Department of Cardiovascular Internal Medicine, Guangzhou Eighth People's Hospital, Guanzhou Medical University, Baiyun District, Guangzhou City, Guangdong Province, China.
- 3. Department of Hepatological Surgery, Guangzhou Eighth People's Hospital, Guanzhou Medical University, Baiyun District, Guangzhou City, Guangdong Province, China.
- 4. Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Gulou District, Nanjing City, Jiangsu Province, China. [email protected].
- 5. Physical Examination Center, Guangzhou Eighth People's Hospital, Guanzhou Medical University, Baiyun District, Guangzhou City, Guangdong Province, China. [email protected].
- # Contributed equally.
Objective: The occurrence of nephrotoxicity induced by Vancomycin (Van) has been documented in both experimental models and clinical patients. In this study, we aimed to identify potential gene targets by utilizing Gene Expression Omnibus (GEO) datasets and a Van-induced mouse model.
Methods: Differentially expressed genes (DEGs) were filtered using GEO datasets (GSE7793), and a mouse model of nephrotoxicity was established by administering Van (400 mg/kg/day for 7 days) via intravenous injection.
Results: A total of 485 DEGs were identified, consisting of 335 upregulated and 150 downregulated genes, based on the criteria of |Log2(fold change)| > 2 and p.adj < 0.05. Through the analysis of protein-protein interaction (PPI) networks, 15 hub genes were identified, suggesting that the kininogen 1 (Kng1)/Bradykinin Receptor B2 (Bdkrb2) system may play a central role in Van-induced nephrotoxicity. In our mouse model, Van treatment resulted in cell Apoptosis and tubular injury in the kidney. The mRNA expression levels of Kng1 and Bdkrb2 were significantly increased in Van-treated mice compared with normal mice. Furthermore, we found that absinthin acts as a potential inhibitor of Bdkrb2 and effectively ameliorates Van-induced podocyte Apoptosis and reduces the levels of renal injury biomarkers, including serum creatinine (Cre), blood urea nitrogen (BUN), and malondialdehyde (MDA).
Conclusions: Our findings suggest that targeting Kng1/Bdkrb2 may offer a potential therapeutic strategy for mitigating Van-induced nephrotoxicity. These findings suggest that absinthin may serve as a renal protective agent against drug-induced nephrotoxicity.