CCT2 Promotes Prostate Cancer Progression Through EIF3F-Dependent Stabilization of FASN

  • Adv Sci (Weinh). 2026 Jun 3:e75915. doi: 10.1002/advs.75915.
Shun Xu  1  2  3 ,  Yifan Zhang  1  2  3 ,  Haolin Li  4 ,  Shengyu Zhao  1  2  3 ,  Xiaoran Dai  5 ,  Qili Xu  1  2  3 ,  Mintian Fei  1  2  3 ,  Chun Li  1  2  3 ,  Zhihui Zou  1  2  3 ,  Baojun Wang  1  2  3 ,  Li Zhang  1  2  3 ,  Hui Wang  1  2  3 ,  Ligang Zhang  1  2  3 ,  Chaozhao Liang  1  2  3
Affiliations
  • 1. Department of Urology, the First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, Anhui, China.
  • 2. Institute of Urology, Anhui Medical University, Hefei, Anhui, China.
  • 3. Anhui Province Key Laboratory of Urological and Andrological Diseases Research and Medical Transformation, Anhui Medical University, Hefei, Anhui, China.
  • 4. Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
  • 5. Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
PMID: 42231807 DOI: 10.1002/advs.75915
Abstract

Prostate Cancer (PCa) is increasingly recognized to be driven by dysregulated lipid metabolism. Although fatty acid synthase (FASN) is highly expressed in PCa, the mechanisms governing FASN protein stability and its functional integration into oncogenic lipid metabolism remain poorly defined. In this study, we identified chaperonin-containing TCP1 subunit 2 (CCT2) as a key oncogenic regulator that promotes lipid synthesis and enhances malignant phenotypes both in vitro and in vivo. Mechanistically, CCT2 transcription is upregulated by the transcription factor Forkhead Box A1 (FOXA1); the CCT2 protein interacts with eukaryotic translation initiation factor 3 subunit F (EIF3F) and FASN to facilitate the assembly of a CCT2/EIF3F/FASN ternary complex. This complex enhances the EIF3F-mediated deubiquitination of FASN, increasing FASN stability and lipid synthesis, and accelerating tumor progression. Either orlistat-mediated FASN inhibition or Y043-8015-induced disruption of the CCT2-EIF3F interaction effectively suppressed CCT2-driven tumor progression in vivo. Importantly, combined treatment produced synergistic antitumor effects, significantly reducing tumor growth and metastatic burden across multiple in vivo models, including isograft and patient-derived xenograft models. This study reveals that CCT2 promotes lipid metabolic reprogramming and tumor progression in prostate Cancer by cooperating with EIF3F to stabilize FASN, highlighting the CCT2-EIF3F-FASN axis as a potential target for metabolic intervention.

Keywords
CCT2; EIF3F; FASN; lipid metabolism reprogramming; prostate cancer.
Products