2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. PYCR1-IN-1

PYCR1-IN-1 (compound 4) is a pyrroline-5-carboxylate reductase 1 (PYCR1) inhibitor with an IC50 of 8.8 µM. PYCR1-IN-1 has anticancer effects.

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PYCR1-IN-1

PYCR1-IN-1 構造式

CAS 番号 : 709-85-3

容量 価格（税別） 在庫状況 数量
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Liquid
10 mg $50 在庫あり
25 mg $75 在庫あり
50 mg $120 在庫あり
100 mg $195 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 2 publication(s) in Google Scholar

PYCR1-IN-1 関連抗体

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DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

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RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

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製品説明

PYCR1-IN-1 (compound 4) is a pyrroline-5-carboxylate reductase 1 (PYCR1) inhibitor with an IC50 of 8.8 µM. PYCR1-IN-1 has anticancer effects[1].

IC50 & Target

IC50: 8.8 µM (PYCR1)[1]
体外実験

PYCR1-IN-1 (0-100 μM, 24 h) decreases the proline level, inhibits the proliferation of human breast cancer cell SUM-159-PT and MDA-MB-231 without additionally supplement of proline (inhibition rate=30-40%)[1].
PYCR1-IN-1 inhibits pyrroline-5-carboxylate reductase (IC50=8.7 μM with the utilization of NADPH as electron donor), interfers with the proline/pyrroline-5-carboxylate cycle, inhibiting thereby the growth of cancer cell[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PYCR1-IN-1 関連抗体

Cell Proliferation Assay[1]

Cell Line: SUM-159-PT and MDA-MB-231
Concentration: 10 μM
Incubation Time: 1-5 days
Result: Inhibited the cell proliferation.
分子量

238.13

分子式

C11H12BrN
CAS 番号
Appearance

Liquid (Density: 1.330±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

C#CCN(C)CC1=CC=C(Br)C=C1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (419.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1994 mL 20.9969 mL 41.9939 mL
5 mM 0.8399 mL 4.1994 mL 8.3988 mL
10 mM 0.4199 mL 2.0997 mL 4.1994 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (21.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.65%

COA (244 KB) HNMR (219 KB) LCMS (94 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1994 mL 20.9969 mL 41.9939 mL 104.9847 mL
5 mM 0.8399 mL 4.1994 mL 8.3988 mL 20.9969 mL
10 mM 0.4199 mL 2.0997 mL 4.1994 mL 10.4985 mL
15 mM 0.2800 mL 1.3998 mL 2.7996 mL 6.9990 mL
20 mM 0.2100 mL 1.0498 mL 2.0997 mL 5.2492 mL
25 mM 0.1680 mL 0.8399 mL 1.6798 mL 4.1994 mL
30 mM 0.1400 mL 0.6999 mL 1.3998 mL 3.4995 mL
40 mM 0.1050 mL 0.5249 mL 1.0498 mL 2.6246 mL
50 mM 0.0840 mL 0.4199 mL 0.8399 mL 2.0997 mL
60 mM 0.0700 mL 0.3499 mL 0.6999 mL 1.7497 mL
80 mM 0.0525 mL 0.2625 mL 0.5249 mL 1.3123 mL
100 mM 0.0420 mL 0.2100 mL 0.4199 mL 1.0498 mL
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PYCR1-IN-1 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PYCR1-IN-1709-85-3DNA/RNA SynthesisPYCR1 inhibitorprolineanticancerInhibitorinhibitorinhibit

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製品名:
PYCR1-IN-1
製品番号:
HY-126271
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