Pyrene phosphoramidite dU
Pyrene phosphoramidite Du is a click chemistry reagent containing pyrene groups. The pyrene group in Pyrene phosphoramidite Du can be inserted into DNA with strong blue fluorescence.
For research use only. We do not sell to patients.
- CAS No.: 199920-17-7
- Formula: C57H55N4O8P
- Molecular Weight:955.04
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
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Bz-rC PhosphoramiditePhosphinamide Monomer
Biological Activity
Description
Chemical Information
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CAS No. 199920-17-7
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Molecular Weight 955.04
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Formula C57H55N4O8P
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SMILES
COC1=CC=C(C(C2=CC=C(OC)C=C2)(OC[C@@H]3[C@@H](OP(N(C(C)C)C(C)C)OCCC#N)C[C@H](N(C(N4)=O)C=C(C#CC5=C(C=C6)C7=C8C6=CC=CC8=CC=C7C=C5)C4=O)O3)C9=CC=CC=C9)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pyrene phosphoramidite dU Related Classifications
HY-151750 Related Classifications
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