1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) ADAMs/ADAMTSs
  4. ADAM17/TACE
  5. ADAM17/TACE Protein, Rat (HEK293, His)

ADAM17/TACE Protein, Rat (HEK293, His)

Cat. No.: HY-P72815
COA Handling Instructions

ADAM17/TACE Protein, a pivotal enzyme, cleaves TNF-alpha and activates various cell-surface proteins like p75 TNF-receptor. It also mediates the cleavage of Notch, activates the Notch pathway, and contributes to hemostasis by shedding GP1BA. ADAM17/TACE further facilitates the cleavage of LAG3, IL6R, and FCGR3A, showcasing its significance in diverse cellular functions. ADAM17/TACE Protein, Rat (HEK293, His) is the recombinant rat-derived ADAM17/TACE protein, expressed by HEK293 , with C-10*His labeled tag. The total length of ADAM17/TACE Protein, Rat (HEK293, His) is 546 a.a., with molecular weight of 57 & 20 kDa, respectively.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
2 μg $80 In-stock
10 μg $225 In-stock
50 μg $630 In-stock
100 μg $1070 In-stock
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ADAM17/TACE Protein, a pivotal enzyme, cleaves TNF-alpha and activates various cell-surface proteins like p75 TNF-receptor. It also mediates the cleavage of Notch, activates the Notch pathway, and contributes to hemostasis by shedding GP1BA. ADAM17/TACE further facilitates the cleavage of LAG3, IL6R, and FCGR3A, showcasing its significance in diverse cellular functions. ADAM17/TACE Protein, Rat (HEK293, His) is the recombinant rat-derived ADAM17/TACE protein, expressed by HEK293 , with C-10*His labeled tag. The total length of ADAM17/TACE Protein, Rat (HEK293, His) is 546 a.a., with molecular weight of 57 & 20 kDa, respectively.

Background

ADAM17, also known as TACE, serves as a pivotal enzyme in diverse cellular processes, notably cleaving the membrane-bound precursor of TNF-alpha to its mature soluble form. Beyond its role in TNF-alpha processing, ADAM17 exhibits a broad substrate specificity, orchestrating the proteolytic release of various cell-surface proteins such as p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. Moreover, ADAM17 acts as an activator of the Notch pathway by mediating the cleavage of Notch, generating the membrane-associated intermediate fragment known as Notch extracellular truncation (NEXT). Its involvement extends to hemostasis through shedding the platelet glycoprotein Ib alpha chain (GP1BA) and mediating the cleavage of LAG3, IL6R, and FCGR3A, highlighting its significance in diverse cellular functions.

Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 and the specific activity is 250-2200 pmol/min/µg.

Species

Rat

Source

HEK293

Tag

C-10*His

Accession

Q9Z1K9 (P18-D563)

Gene ID
Molecular Construction
N-term
ADAM17 (P18-D563)
Accession # Q9Z1K9
10*His
C-term
Synonyms
Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; TNF-alpha convertase; CD156b; Tace
AA Sequence

PRPPEEPGSGSHLRLEKLDSLLSDYDILSLSNIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQDFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSLDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPGVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGDAED

Molecular Weight

57&20 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ADAM17/TACE Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ADAM17/TACE Protein, Rat (HEK293, His)
Cat. No.:
HY-P72815
Quantity:
MCE Japan Authorized Agent: