ADAMTS13 Protein, Human (CHO, His)

The ADAMTS13 protein, a metalloproteinase, has a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 motif. It cleaves von Willebrand Factor and defects in this gene cause thrombotic thrombocytopenic purpura. Alternative splicing generates multiple transcript variants. ADAMTS13 is broadly expressed in various tissues, including the liver, testis, and 24 other tissues. ADAMTS13 Protein, Human (CHO, His) is the recombinant human-derived ADAMTS13, expressed by CHO, with C-10*His labeled tag. The total length of ADAMTS13 Protein, Human (CHO, His) is 655 a.a..

The ADAMTS13 protein, a member of the metalloproteinase family, contains distinct regions including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. This enzyme specifically cleaves von Willebrand Factor (vWF) and defects in this gene are associated with thrombotic thrombocytopenic purpura. Multiple transcript variants are generated through alternative splicing. Additionally, the ADAMTS13 protein exhibits broad expression in various tissues, including the liver, testis, and 24 other tissues.

Measured by its ability to cleave 4 µM fluorogenic peptide substrate, FRETS-VWF73 (HY-P5354) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >300 pmol/min/µg.

Human

CHO

C-10*His

NP_620594 (Q34-W688)

11093  [NCBI]

Partial

QQSCLQALEPQAVSSYLSPGAPLKGRPPSPGFQRQRQRQRRAAGGILHLELLVAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPSLGAQFRVHLVKMVILTEPEGAPNITANLTSSLLSVCGWSQTINPEDDTDPGHADLVLYITRFDLELPDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLGVTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASDGAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDPPRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKAVACTFAREHLDMCQALSCHTDPLDQSSCSRLLVPLLDGTECGVEKWCSKGRCRSLVELTPIAAVHGRWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFGGRACVGADLQAEMCNTQACEKTQLEFMSQQCARTDGQPLRSSPGGASFYHWGAAVPHSQGDALCRHMCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTLSLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDNSTCSPRKGSFTAGRAREYVTFLTVTPNLTSVYIANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPSLLEDGRVEYRVALTEDRLPRLEEIRIWGPLQEDADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQAWVW

Approximately 90 kDa, based on SDS-PAGE under reducing condition, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.