Copy Link Print Information

C1QTNF1/CTRP1 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

C1QTNF1/CTRP1 Protein, Human (HEK293, His) is a recombinant human C1q/TNF-related protein 1 (CTRP1) produced in HEK293 cells, with His tag. CTRP1 is an adipokine that is mainly expressed in adipose-derived cells and adipocytes.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for C1QTNF1/CTRP1 Protein, Human (HEK293, His)
  • Experimental Validation Results for C1QTNF1/CTRP1 Protein, Human (HEK293, His)
  • C1QTNF1/CTRP1 Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

C1QTNF1/CTRP1 Protein, Human (HEK293, His) is a recombinant human C1q/TNF-related protein 1 (CTRP1) produced in HEK293 cells, with His tag. CTRP1 is an adipokine that is mainly expressed in adipose-derived cells and adipocytes[1].

Background

CTRP1 plays an important role in regulating body energy homeostasis and sensitivity to insulin. High levels of circulating CTRP1 are associated with metabolic syndrome, congestive heart disease, and hypertension[1].

Verified Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized Human BAI3 (HY-P7675) at 2 μg/mL (100 μL/well) can bind Biotinylated Human C1qTNF1. The ED50 for this effect is 1-8 µg/mL.
2. Immobilized Human BAI3 (HY-P7675) at 4 μg/mL (100 μL/well) can bind Biotinylated Human C1qTNF1. The ED50 for this effect is 2.0-3.5 µg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human BAI3 at 2 μg/mL (100 μL/well) can bind Biotinylated Human C1qTNF1, The ED50 for this effect is 1.533 µg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9BXJ1-1 (R26-P281)

  • Gene ID
    114897  [NCBI]
  • Molecular Construction
    • N-term
    • C1QTNF1 (R26-P281)
      Accession # Q9BXJ1-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    C1QTNF1; Complement C1q Tumor Necrosis Factor-Related Protein 1; C1q And TNF Related 1; C1q And Tumor Necrosis Factor Related Protein 1; CTRP1; G Protein Coupled Receptor Interacting Protein; GIP; G Protein-Coupled Receptor-Interacting Protein; ZSIG37; FL

  • AA Sequence

    RVPHVQGEQQEWEGTEELPSPPDHAERAEEQHEKYRPSQDQGLPASRCLRCCDPGTSMYPATAVPQINITILKGEKGDRGDRGLQGKYGKTGSAGARGHTGPKGQKGSMGAPGERCKSHYAAFSVGRKKPMHSNHYYQTVIFDTEFVNLYDHFNMFTGKFYCYVPGLYFFSLNVHTWNQKETYLHIMKNEEEVVILFAQVGDRSIMQSQSLMLELREQDQVWVRLYKGERENAIFSEELDTYITFSGYLVKHATEP

  • Molecular Weight

    Approximately 37 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Joanna Dorota Janowska. C1q/TNF-related Protein 1, a Multifunctional Adipokine: An Overview of Current Data. Am J Med Sci. 2020 Sep;360(3):222-228. [Content Brief]

[2]. Linhui Shen, et al. Association of C1q/TNF-related protein-1 (CTRP1) serum levels with coronary artery disease. J Int Med Res. 2019 Jun; 47(6): 2571-2579. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human BAI3 at 2 μg/mL (100 μL/well) can bind Biotinylated Human C1qTNF1, The ED50 for this effect is 1.533 µg/mL.

  • C1QTNF1/CTRP1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for C1QTNF1/CTRP1 Protein, Human (HEK293, His)
  • Experimental Validation Results for C1QTNF1/CTRP1 Protein, Human (HEK293, His)
  • C1QTNF1/CTRP1 Protein, Human (HEK293, His) Product Image