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PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His)

Cat. No.: HY-P74290
Handling Instructions

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His) is the recombinant human-derived PTPRC/CD45RAB protein, expressed by HEK293 , with C-His labeled tag. The total length of PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His) is 529 a.a., with molecular weight of ~57.4 kDa.

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Description

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His) is the recombinant human-derived PTPRC/CD45RAB protein, expressed by HEK293 , with C-His labeled tag. The total length of PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His) is 529 a.a., with molecular weight of ~57.4 kDa.

Background

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, also known as CD45, is a transmembrane glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation.
PTPRC contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.
PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling as PTPRC positive regulate T-cell coactivation upon binding to DPP4, recruiting and dephosphorylating SKAP1 and FYN. PTPRC also dephosphorylates LYN, and thereby modulates LYN activity.
PTPRC functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
PTPRC gene has many alternatively spliced transcripts variants, which encode distinct isoforms[1][2][3].

Species

Human

Source

HEK293

Tag

C-His

Accession

P08575-5 (M1-K529)

Gene ID
Molecular Construction
N-term
PTPRC (M1-K529)
Accession # P08575-5
His
C-term
Synonyms
Receptor-type tyrosine-protein phosphatase C; L-CA; T200; PTPRC; CD45
Molecular Weight

Approximately 57.4 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PTPRC/CD45RAB Protein, Human (529a.a, HEK293, His)
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HY-P74290
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