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  5. CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi)

CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi)

Cat. No.: HY-P72926
COA Handling Instructions

CD64 protein, a high affinity receptor, mediates IgG effector functions, triggering antibody-dependent cellular cytotoxicity (ADCC). It interacts with IGHG1 and forms a signaling complex with FCERG1, contributing to immune responses. CD64 also interacts with FLNA, EPB41L2, LAT, PPL, HCK, and LYN to prevent degradation of FCGR1A and regulate immune function. CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is the recombinant human-derived CD64 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is 272 a.a., with molecular weight of 34-68 kDa.

For research use only. We do not sell to patients.

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Description

CD64 protein, a high affinity receptor, mediates IgG effector functions, triggering antibody-dependent cellular cytotoxicity (ADCC). It interacts with IGHG1 and forms a signaling complex with FCERG1, contributing to immune responses. CD64 also interacts with FLNA, EPB41L2, LAT, PPL, HCK, and LYN to prevent degradation of FCGR1A and regulate immune function. CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is the recombinant human-derived CD64 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi) is 272 a.a., with molecular weight of 34-68 kDa.

Background

CD64 protein is a high affinity receptor for the Fc region of immunoglobulins gamma. It plays a role in both innate and adaptive immune responses. CD64 mediates IgG effector functions on monocytes, triggering antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells. It interacts with IGHG1 and forms a functional signaling complex with FCERG1. CD64 also interacts with FLNA, preventing degradation of FCGR1A. Additionally, it interacts with EPB41L2, LAT, PPL, HCK, and LYN, contributing to its diverse functions in immune regulation.

Biological Activity

1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His & AVI Tag), Biotinylated, the EC50 is 1-3 ng/mL.
2.Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 5 nM as determined in SPR assay (Biacore T200).

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

P12314 (Q16-T287)

Gene ID
Synonyms
High affinity immunoglobulin gamma Fc receptor I; Fcgr1; FcRI; CD64
AA Sequence

MWFLTTLLLWVPVDGQVDTTKAviTLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPT

Molecular Weight

34-68 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD64 Protein, Human (Biotinylated, 272a.a, HEK293, His-Avi)
Cat. No.:
HY-P72926
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