CDK12-CCNK Heterodimer Protein, Human (Active, 658-1490, sf9, GST)
The CDK12 protein is a cyclin-dependent kinase that critically phosphorylates the C-terminal domain of POLR2A, thereby regulating transcription elongation. It selectively targets "Ser-5" phosphorylated at "Ser-7" in the CTD repeat and can also target "Ser-2". CDK12-CCNK Heterodimer Protein, Human (658-1490, sf9, GST) is the recombinant human-derived CDK12-CCNK, expressed by Sf9 insect cells, with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The CDK12 protein is a cyclin-dependent kinase that critically phosphorylates the C-terminal domain of POLR2A, thereby regulating transcription elongation. It selectively targets "Ser-5" phosphorylated at "Ser-7" in the CTD repeat and can also target "Ser-2". CDK12-CCNK Heterodimer Protein, Human (658-1490, sf9, GST) is the recombinant human-derived CDK12-CCNK, expressed by Sf9 insect cells, with N-GST labeled tag.
Background
The CDK12-CCNK heterodimer protein functions as a cyclin-dependent kinase, playing a crucial role in transcription elongation by phosphorylating the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A). This kinase is a key regulator of gene expression, particularly involved in DNA repair processes and the maintenance of genomic stability. Its phosphorylation activity is selective, preferring 'Ser-5' in CTD repeats already phosphorylated at 'Ser-7,' but it can also target 'Ser-2.' CDK12-CCNK is essential for RNA splicing, potentially through the phosphorylation of SRSF1/SF2. Additionally, it participates in the regulation of MAP kinase activity, suggesting its involvement in influencing responses to estrogen inhibitors.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q9NYV4-1 (K658-Y1490)&O75909-3 (K2-R580)
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Gene ID51755&8812
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Protein Length
Partial
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Synonyms
CDK12; KIAA0904; Prev. CRKRS; Cell Division Cycle 2-Related Protein Kinase 7; CRK7; CDC2-Related Protein Kinase 7; Cdc2-Related Kinase, Arginine/Serine-Rich; CDC2 Related Protein Kinase 7; Cell Division Protein Kinase 12; HCDK12; Cyclin-Dependent Kinase 1
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Predicted Molecular Mass
119 kDa (CDK12) & 90 kDa (CCNK)
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Molecular Weight
Approximately 90 kDa (CCNK) & 115-120 kDa (CDK12), based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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