1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2) Serine/Threonine Kinase Proteins
  4. Cyclin-Dependent Kinases (CDKs)
  5. Cyclin-Dependent Kinase 7 (CDK7)
  6. CDK7&CCNH&MNAT1 Protein, Human (sf9, His)

CDK7&CCNH&MNAT1 Protein, Human (sf9, His)

Cat. No.: HY-P75361
Handling Instructions

CDK7, along with regulatory partners CCNH and MNAT1, operates as a crucial serine/threonine kinase in cell cycle regulation and RNA polymerase II-mediated transcription. As the catalytic subunit of the CDK-activating kinase (CAK) complex, CDK7 plays a pivotal role in activating CDK1/cyclin-B and CDK2/cyclins during cell cycle transitions. It phosphorylates key targets, including SPT5/SUPT5H and POLR2A. CDK7, in complex with TFIIH, activates RNA polymerase II for transcript elongation. Its consistent expression and activity contribute to DNA-bound peptide-mediated transcription and cellular growth regulation. CDK7&CCNH&MNAT1 Protein, Human (sf9, His) is the recombinant heterotrimer protein (consiting of CDK7, CCNH, MNAT1), expressed by Sf9 insect cells, with N-His labeled tag. CDK7&CCNH&MNAT1 Protein, Human (sf9, His), has molecular weight of ~25 & 38 & 44 kDa, respectively.

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Description

CDK7, along with regulatory partners CCNH and MNAT1, operates as a crucial serine/threonine kinase in cell cycle regulation and RNA polymerase II-mediated transcription. As the catalytic subunit of the CDK-activating kinase (CAK) complex, CDK7 plays a pivotal role in activating CDK1/cyclin-B and CDK2/cyclins during cell cycle transitions. It phosphorylates key targets, including SPT5/SUPT5H and POLR2A. CDK7, in complex with TFIIH, activates RNA polymerase II for transcript elongation. Its consistent expression and activity contribute to DNA-bound peptide-mediated transcription and cellular growth regulation. CDK7&CCNH&MNAT1 Protein, Human (sf9, His) is the recombinant heterotrimer protein (consiting of CDK7, CCNH, MNAT1), expressed by Sf9 insect cells, with N-His labeled tag. CDK7&CCNH&MNAT1 Protein, Human (sf9, His), has molecular weight of ~25 & 38 & 44 kDa, respectively.

Background

CDK7, in conjunction with its regulatory partners CCNH and MNAT1, functions as a serine/threonine kinase with pivotal roles in cell cycle regulation and RNA polymerase II-mediated transcription. As a catalytic subunit of the CDK-activating kinase (CAK) complex, CDK7 plays a critical role in the activation and formation of CDK1/cyclin-B during the G2-M transition and CDK2/cyclins during the G1-S transition. Its phosphorylation targets include SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6, and CDK11B/CDK11. Through threonine phosphorylation, CAK activates cyclin-associated kinases, thereby regulating cell cycle progression. Moreover, CDK7, when complexed with the core-TFIIH basal transcription factor, facilitates RNA polymerase II activation by serine phosphorylation of the C-terminal domain (CTD) of its large subunit (POLR2A). This activation enables RNA polymerase II to escape the promoter and initiate transcript elongation. CDK7's consistent expression and activity throughout the cell cycle contribute to its involvement in DNA-bound peptides-mediated transcription and cellular growth inhibition. Additionally, in response to DNA damage, CDK7 triggers the activation of p53/TP53, forming a feedback loop where p53/TP53 can subsequently inactivate CDK7, potentially leading to cell cycle arrest, transcriptional shutdown, and cellular recovery or apoptosis.

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

P50613 (A2-F346)&P51946 (Y2-L323)&P51948 (D2-S309)

Gene ID

1022  [NCBI]&902  [NCBI]&4331  [NCBI]

Synonyms
CAK1 Protein; CDKN7 Protein; HCAK Protein; MO15 Protein; p39MO15 Protein; STK1 Protein
Molecular Weight

Approximately 25&38&44 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CDK7&CCNH&MNAT1 Protein, Human (sf9, His)
Cat. No.:
HY-P75361
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