CDK7-CCNH-MNAT1 Protein, Human (Active, sf9, His)
Together with its regulatory partners CCNH and MNAT1, CDK7 functions as an important serine/threonine kinase in cell cycle regulation and RNA polymerase II-mediated transcription. As the catalytic subunit of the CDK-activated kinase (CAK) complex, CDK7 plays a key role in activating CDK1/cyclin-B and CDK2/cyclin during cell cycle transitions. CDK7-CCNH-MNAT1 Protein, Human (sf9, His) is the recombinant human-derived CDK7-CCNH-MNAT1 protein, expressed by Sf9 insect cells , with N-His labeled tag. CDK7-CCNH-MNAT1 Protein, Human (sf9, His), has molecular weight of ~25 & 38 & 44 kDa, respectively.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Together with its regulatory partners CCNH and MNAT1, CDK7 functions as an important serine/threonine kinase in cell cycle regulation and RNA polymerase II-mediated transcription. As the catalytic subunit of the CDK-activated kinase (CAK) complex, CDK7 plays a key role in activating CDK1/cyclin-B and CDK2/cyclin during cell cycle transitions. CDK7-CCNH-MNAT1 Protein, Human (sf9, His) is the recombinant human-derived CDK7-CCNH-MNAT1 protein, expressed by Sf9 insect cells , with N-His labeled tag. CDK7-CCNH-MNAT1 Protein, Human (sf9, His), has molecular weight of ~25 & 38 & 44 kDa, respectively.
Background
CDK7, in conjunction with its regulatory partners CCNH and MNAT1, functions as a serine/threonine kinase with pivotal roles in cell cycle regulation and RNA polymerase II-mediated transcription. As a catalytic subunit of the CDK-activating kinase (CAK) complex, CDK7 plays a critical role in the activation and formation of CDK1/cyclin-B during the G2-M transition and CDK2/cyclins during the G1-S transition. Its phosphorylation targets include SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6, and CDK11B/CDK11. Through threonine phosphorylation, CAK activates cyclin-associated kinases, thereby regulating cell cycle progression. Moreover, CDK7, when complexed with the core-TFIIH basal transcription factor, facilitates RNA polymerase II activation by serine phosphorylation of the C-terminal domain (CTD) of its large subunit (POLR2A). This activation enables RNA polymerase II to escape the promoter and initiate transcript elongation. CDK7's consistent expression and activity throughout the cell cycle contribute to its involvement in DNA-bound peptides-mediated transcription and cellular growth inhibition. Additionally, in response to DNA damage, CDK7 triggers the activation of p53/TP53, forming a feedback loop where p53/TP53 can subsequently inactivate CDK7, potentially leading to cell cycle arrest, transcriptional shutdown, and cellular recovery or apoptosis.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-10*His
-
Synonyms
CDK7; Cyclin-Dependent Kinase 7 (Homolog Of Xenopus MO15 Cdk-Activating Kinase); Cyclin Dependent Kinase 7; Homolog Of Xenopus MO15 Cdk-Activating Kinase; CDKN7; Serine/Threonine Protein Kinase MO15; CAK1; Cyclin-Dependent Kinase 7 Isoform 3; MO15; Serine/Threonine Protein Kinase 1; STK1; Serine/Threonine-Protein Kinase 1; CAK; [RNA-Polymerase]-Subunit Kinase; TFIIH Basal Transcription Factor Complex Kinase Subunit; Serine/Threonine Kinase Stk1; Cell Division Protein Kinase 7; Kinase Subunit Of CAK; Cyclin-Dependent Kinase 7; P39 Mo15; Cyclin-Dependent Kinase 7 (MO15 Homolog, Xenopus Laevis, Cdk-Activating Kinase); P39MO15; CDK-Activating Kinase 1; HCAK; 39 KDa Protein Kinase; CCNH; CycH; Cyclin H; Cyclin-Dependent Kinase-Activating Kinase Complex Subunit; P34; CDK-Activating Kinase Complex Subunit; P37; CAK Complex Subunit; MO15-Associated Protein; CAK; Cyclin-H; MNAT1; CAP35; MNAT1 Component Of CDK Activating Kinase; P36; RNF66; P35; MAT1; Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis); CDK-Activating Kinase Assembly Factor MAT1; Menage A Trois-Like Protein 1 Cyclin H Assembly Factor Isoform 2; CDK7/Cyclin-H Assembly Factor; Menage A Trois-Like Protein 1 Cyclin H Assembly Factor; RING Finger Protein MAT1; Menage A Trois Homolog 1, Cyclin H Assembly Factor; MNAT CDK-Activating Kinase Assembly Factor 1; CDK-Activating Kinase Assembly Factor; MNAT1, CDK Activating Kinase Assembly Factor; Cyclin G1 Interacting Protein; Menage A Trois 1 (CAK Assembly Factor); Cyclin-G1-Interacting Protein; RING Finger Protein 66; TFB3; Menage A Trois
-
Predicted Molecular Mass
41.2 kDa&39.7 kDa&37.9 kDa
-
Molecular Weight
Approximately 25 kDa & 38 kDa & 44 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
