CHGA Protein, Human (P.pastoris, His)
Based on 1 Customer Validation
CHGA protein robustly inhibits glucose-induced insulin release from the pancreas, functioning as a non-competitive nicotinic cholinergic antagonist. It inhibits catecholamine release, displays antibacterial activity, induces mast cell responses, and scavenges free radicals. CHGA may also regulate cardiac function and blood pressure. CHGA Protein, Human (P.pastoris, His) is the recombinant human-derived CHGA protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Human
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CHGA protein robustly inhibits glucose-induced insulin release from the pancreas, functioning as a non-competitive nicotinic cholinergic antagonist. It inhibits catecholamine release, displays antibacterial activity, induces mast cell responses, and scavenges free radicals. CHGA may also regulate cardiac function and blood pressure. CHGA Protein, Human (P.pastoris, His) is the recombinant human-derived CHGA protein, expressed by P. pastoris , with N-His labeled tag.
Background
CHGA protein exerts a strong inhibitory effect on glucose-induced insulin release from the pancreas. It functions as a non-competitive nicotinic cholinergic antagonist, inhibiting catecholamine release from chromaffin cells and noradrenergic neurons. Additionally, CHGA displays antibacterial activity against both Gram-positive (S. aureus and M. luteus) and Gram-negative bacteria (E. coli and P. aeruginosa). Moreover, it has the capacity to induce mast cell migration, degranulation, and the production of cytokines and chemokines. Furthermore, CHGA acts as a potent scavenger of free radicals in vitro and may play a role in the regulation of cardiac function and blood pressure.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized SCG3 (HY-P71279) at 1 μg/mL (100 μL/well) can bind CHGA (HY-P71727). The ED50 for this effect is 0.308 μg/mL.
Technical Parameters
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Species Human
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Source P. pastoris
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Tag N-His
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Accession
P10645 (L19-G457)
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Molecular Construction
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N-term
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His
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CHGA (L19-G457)
Accession # P10645 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CHGA; Betagranin (N-Terminal Fragment Of Chromogranin A); Chromogranin A; Pancreastatin; Chromogranin-A; Chromofungin; PHE5; Vasostatin; PHES; Parastatin; Parathyroid Secretory Protein 1; Catestatin; Pituitary Secretory Protein I; CGA; SP-I; CgA
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AA Sequence
LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG
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Predicted Molecular Mass
50.9 kDa
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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