Cyclin E Protein, Human (sf9)
Based on 1 Customer Validation
Cyclin E controls the G1/S transition of the cell cycle. Cyclin E Protein, Human (sf9) is the recombinant human-derived Cyclin E, expressed by Sf9 insect cells , with tag Free labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Cyclin E controls the G1/S transition of the cell cycle. Cyclin E Protein, Human (sf9) is the recombinant human-derived Cyclin E, expressed by Sf9 insect cells , with tag Free labeled tag.
Cyclin E, an integral protein in the intricate regulation of the cell cycle, plays a pivotal role in governing the transition from the G1 phase to the S phase. Through its interaction with the CDK2 protein kinase, Cyclin E forms a dynamic serine/threonine kinase holoenzyme complex, with the cyclin subunit conferring substrate specificity to this crucial enzymatic assembly. Cyclin E is part of various complexes, including those involving CDK2, CABLES1, and CCNA1, as well as a complex comprising UHRF2, CDK2, and CCNE1. The interaction with UHRF2 leads to the ubiquitination of Cyclin E independently of its phosphorylation status, showcasing the intricate regulatory mechanisms at play. Additionally, Cyclin E engages in interactions with INCA1, further contributing to its multifaceted involvement in cell cycle control.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag Tag Free
-
Accession
P24864 (M1-A410)
-
Molecular Construction
-
N-term
-
Cyclin E (M1-A410)
Accession # P24864 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
CCNE1; Cyclin E Variant Ex7del; Prev. CCNE; Cyclin Et; G1/S-Specific Cyclin-E1; PCCNE1; Cyclin E Variant Ex5del; Cyclin E1; Cyclin Es
-
AA Sequence
MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
-
Molecular Weight
Approximately 47.3 kDa
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES(pH 7.5), 300 mM NaCl, 5% glycerol.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (237 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
