DPYS Protein, Human (sf9)
The DPYS protein plays a key role in the reductive pyrimidine degradation pathway by catalyzing the second step involving the reversible hydrolysis of dihydropyrimidines. Its enzymatic activity extends to the ring opening of 5,6-dihydrouracil, leading to the formation of N-carbamoyl-alanine, and the conversion of 5,6-dihydrothymine to N-carbamoyl-aminoisobutylene acid ester. DPYS Protein, Human (sf9) is the recombinant human-derived DPYS protein, expressed by Sf9 insect cells, with tag free.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The DPYS protein plays a key role in the reductive pyrimidine degradation pathway by catalyzing the second step involving the reversible hydrolysis of dihydropyrimidines. Its enzymatic activity extends to the ring opening of 5,6-dihydrouracil, leading to the formation of N-carbamoyl-alanine, and the conversion of 5,6-dihydrothymine to N-carbamoyl-aminoisobutylene acid ester. DPYS Protein, Human (sf9) is the recombinant human-derived DPYS protein, expressed by Sf9 insect cells, with tag free.
Dihydropyrimidinase (DPYS) is an enzyme that plays a crucial role in the reductive pyrimidine degradation pathway, catalyzing the reversible hydrolytic ring opening of dihydropyrimidines. Specifically, DPYS can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. This enzymatic activity is a key step in the catabolism of pyrimidine derivatives, contributing to the breakdown of nucleotide and nucleic acid metabolites. The versatility of DPYS in the hydrolytic ring opening of dihydropyrimidines highlights its significance in maintaining pyrimidine homeostasis and the recycling of pyrimidine-related compounds within the cell. Understanding the functions of DPYS provides insights into the regulation of pyrimidine metabolism and its implications for cellular processes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q14117 (N-G&P, M1-P519)
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Molecular Construction
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N-term
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Gly-Pro
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DPYS (M1-P519)
Accession # Q14117 -
C-term
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Protein Length
Full Length
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Synonyms
DPYS; Dihydropyrimidine Amidohydrolase; Dihydropyrimidinase; Hydantoinase; DHPase; DHP
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Predicted Molecular Mass
56.8 kDa
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Molecular Weight
Approximately 54 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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