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FLRT3 Protein, Human (HEK293, His)

Cat. No.: HY-P70924
COA Handling Instructions

The FLRT3 protein exhibits multiple roles in cellular processes, affecting cell-cell adhesion, migration, and axonal guidance, with context-dependent attraction or repulsion. FLRT3 is critical for the spatial organization of brain neurons and also contributes to retinal blood vessel development. FLRT3 Protein, Human (HEK293, His) is the recombinant human-derived FLRT3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of FLRT3 Protein, Human (HEK293, His) is 500 a.a., with molecular weight of 60-110 kDa.

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Description

The FLRT3 protein exhibits multiple roles in cellular processes, affecting cell-cell adhesion, migration, and axonal guidance, with context-dependent attraction or repulsion. FLRT3 is critical for the spatial organization of brain neurons and also contributes to retinal blood vessel development. FLRT3 Protein, Human (HEK293, His) is the recombinant human-derived FLRT3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of FLRT3 Protein, Human (HEK293, His) is 500 a.a., with molecular weight of 60-110 kDa.

Background

FLRT3 protein plays a multifaceted role in cellular processes such as cell-cell adhesion, cell migration, and axon guidance, demonstrating either attractive or repulsive effects contingent on its interaction partners. Crucially involved in the spatial organization of brain neurons, FLRT3 also contributes to vascular development in the retina. Through interaction with ADGRL3 and potentially other latrophilins on adjacent cells, FLRT3 participates in cell-cell adhesion. Its interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1, while also facilitating axon growth cone collapse and playing a repulsive role in neuron guidance via UNC5B and potentially other UNC-5 family members. FLRT3 promotes neurite outgrowth and mediates cell-cell contacts that enhance both neurite number and length. Additionally, it regulates the density of glutamatergic synapses and is implicated in fibroblast growth factor-mediated signaling cascades. Essential for normal morphogenesis during embryonic development, FLRT3 contributes to processes like ventral closure, headfold fusion, and definitive endoderm migration, as well as the formation and maintenance of a normal basement membrane and anterior visceral endoderm. FLRT3 forms monomers, homodimers, and self-associates through leucine-rich repeats, creating homooligomers. Interactions include those with FGFR1, ADGRL1/LPHN1, LPHN2, ADGRL3, UNC5B, UNC5D, and ROBO1.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q9NZU0 (K29-P528)

Gene ID
Molecular Construction
N-term
FLRT3 (K29-P528)
Accession # Q9NZU0
6*His
C-term
Synonyms
Leucine-Rich Repeat Transmembrane Protein FLRT3; Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 3; FLRT3; KIAA1469
AA Sequence

KSCPSVCRCDAGFIYCNDRFLTSIPTGIPEDATTLYLQNNQINNAGIPSDLKNLLKVERIYLYHNSLDEFPTNLPKYVKELHLQENNIRTITYDSLSKIPYLEELHLDDNSVSAVSIEEGAFRDSNYLRLLFLSRNHLSTIPWGLPRTIEELRLDDNRISTISSPSLQGLTSLKRLVLDGNLLNNHGLGDKVFFNLVNLTELSLVRNSLTAAPVNLPGTNLRKLYLQDNHINRVPPNAFSYLRQLYRLDMSNNNLSNLPQGIFDDLDNITQLILRNNPWYCGCKMKWVRDWLQSLPVKVNVRGLMCQAPEKVRGMAIKDLNAELFDCKDSGIVSTIQITTAIPNTVYPAQGQWPAPVTKQPDIKNPKLTKDHQTTGSPSRKTITITVKSVTSDTIHISWKLALPMTALRLSWLKLGHSPAFGSITETIVTGERSEYLVTALEPDSPYKVCMVPMETSNLYLFDETPVCIETETAPLRMYNPTTTLNREQEKEPYKNPNLP

Molecular Weight

60-110 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

FLRT3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FLRT3 Protein, Human (HEK293, His)
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HY-P70924
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